当前位置: X-MOL 学术ChemBioChem › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Towards Efficient Nonenzymatic DNA Ligation: Comparing Key Parameters for Maximizing Ligation Rates and Yields with Carbodiimide Activation.
ChemBioChem ( IF 2.6 ) Pub Date : 2020-07-23 , DOI: 10.1002/cbic.202000335
Chiamaka Obianyor 1 , Gary Newnam 2 , Bryce E Clifton 2 , Martha A Grover 1 , Nicholas V Hud 2
Affiliation  

Get the most out of your ligation: Nonenzymatic DNA ligation with carbodiimides often results in low ligation yields. Using a simple bimolecular ligating framework, we systematically identified parameters that can be optimized to achieve near‐quantitative ligation.
image


中文翻译:

迈向高效的非酶DNA连接:比较关键参数以最大化碳二亚胺活化的连接速率和产率。

充分利用连接:非酶DNA与碳二亚胺的连接通常会导致连接产量低。使用简单的双分子连接框架,我们系统地确定了可以优化以实现接近定量连接的参数。
图片
更新日期:2020-07-23
down
wechat
bug