The detection of the markers of Cannabis consumption in biological specimens is an important task for drug testing laboratories in varous contexts. A simple assay combining salting‐out assisted liquid–liquid extraction sample preparation and LC–MS/MS analysis was applied to the measurement of Δ⁹‐tetrahydrocannabinol, 11‐nor‐9‐carboxy‐Δ⁹‐tetrahydrocannabinol (THC‐COOH), 11‐hydroxy‐Δ⁹‐tetrahydrocannabinol, cannabinol and cannabidiol concentrations in 100 μl plasma specimens. The assay had linearity of 1–100 ng ml⁻¹ for THC‐COOH and 0.5–50 ng ml⁻¹ for the other tested cannabinoids. Assay validation criteria were fulfilled. Extraction yields (88.7–97.3%) and internal‐standard correct matrix effects (−9.6 to +5.4%) were acceptable. The assay was applied to 238 clinical specimens from trauma patients, with 19 samples presenting quantifiable concentrations of at least one of the target compounds. The developed assay is a simple and efficient strategy for simultaneous measurement of Δ⁹‐tetrahydrocannabinol, THC‐COOH, 11‐hydroxy‐Δ⁹‐tetrahydrocannabinol, cannabinol and cannabidiol concentrations in plasma specimens.

中文翻译：

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使用盐析辅助液-液萃取，然后进行LC-MS / MS分析，测定血浆中的大麻素。

在各种情况下，检测生物样本中大麻消费的标志是药物测试实验室的一项重要任务。一个简单的测定法组合盐析，辅助液-液萃取的样品制备和LC-MS / MS分析应用于Δ的测量⁹四氢大麻酚，11-也不-9-羧基- Δ ⁹ -四氢大麻酚（THC-COOH）， 11-羟基Δ ⁹ -四氢大麻酚，大麻酚和大麻二酚的浓度在100μl血浆样本。该测定法对THC-COOH的线性为1–100 ng ml ^-1，而线性为0.5–50 ng ml ^-1用于其他经过测试的大麻素。满足测定验证标准。提取率（88.7–97.3％）和内标正确的基质效应（−9.6至+ 5.4％）是可以接受的。该测定法应用于来自创伤患者的238个临床标本，其中19个样品的定量浓度至少为一种目标化合物。所开发的测定是对于Δ的同时测量的简单且有效的策略⁹四氢大麻酚，THC-COOH，11-羟基Δ ⁹ -四氢大麻酚，在血浆样本大麻酚和大麻二酚的浓度。