Journal of Trace Elements in Medicine and Biology ( IF 3.6 ) Pub Date : 2020-07-24 , DOI: 10.1016/j.jtemb.2020.126630 Shradhda B Nadhe 1 , Madhumita S Tawre 1 , Sonia Agrawal 2 , Balu A Chopade 3 , Dhiman Sarkar 2 , Karishma Pardesi 1
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Background
Biogenic nanoparticles are gaining attention due to their low toxicity and numerous biomedical applications. Present study aimed to compare the potential anticancer activity of two biogenic silver nanoparticles (bAgNPs and pAgNPs) against human cervical cancer cell lines (HeLa).
Methods
bAgNPs were synthesized using Acinetobacter sp. whereas pAgNPs were synthesized using aqueous root extract of Curcuma aromatica. Effect of these nanoparticles on HeLa cells viability was studied using MTT assay and colony formation assay. Anticancer potential was determined using fluorescence microscopy and flow cytometry studies. Bio-compatibility studies were performed against peripheral blood mononuclear cells (PBMCs).
Results
Both the nanoparticles showed 50 % viability of peripheral blood mononuclear cells (PBMCs) when used at high concentration (200 μg/mL). IC50 for bAgNPs and pAgNPs against HeLa cells were 17.4 and 14 μg/mL respectively. Colony formation ability of Hela cells was reduced on treatment with both nanoparticles. Acridine orange and ethidium bromide staining demonstrated that bAgNPs were cytostatic whereas pAgNPs were apoptotic. JC-1 dye staining revealed that the mitochondrial membrane potential was affected on treatment with pAgNPs while it remained unchanged on bAgNPs treatment. Flow cytometry confirmed cell cycle arrest in HeLa cells on treatment with nanoparticles further leading to apoptosis in case of pAgNPs. About 77 and 58 % HeLa cells were found in subG1 phase on treatment with bAgNPs and pAgNPs respectively. bAgNPs showed cytostatic effect on HeLa cells arresting the cell growth in subG1 phase, whereas, pAgNPs triggered death of HeLa cells through mitochondrial membrane potential impairment and apoptosis.
Conclusion
Overall, bAgNPs and pAgNPs could be safe and showed potential to be used as anticancer nano-antibiotics against human cervical cancer cells.
中文翻译：
使用不动杆菌合成的 AgNPs 的抗癌潜力。和姜黄对 HeLa 细胞系的影响：一项比较研究。
背景
生物纳米粒子因其低毒性和众多生物医学应用而受到关注。本研究旨在比较两种生物银纳米粒子（bAgNPs 和 pAgNPs）对人宫颈癌细胞系（HeLa）的潜在抗癌活性。
方法
bAgNPs 是使用不动杆菌合成的。而 pAgNPs 是使用姜黄的水根提取物合成的。使用 MTT 测定和集落形成测定研究这些纳米颗粒对 HeLa 细胞活力的影响。使用荧光显微镜和流式细胞术研究确定抗癌潜力。针对外周血单核细胞 (PBMC) 进行了生物相容性研究。
结果
当以高浓度 (200 μg/mL) 使用时，两种纳米颗粒都显示出 50% 的外周血单核细胞 (PBMC) 存活率。集成电路50bAgNPs 和 pAgNPs 对 HeLa 细胞的抑制分别为 17.4 和 14 μg/mL。用两种纳米颗粒处理后，Hela 细胞的集落形成能力降低。吖啶橙和溴化乙锭染色表明 bAgNPs 是细胞抑制性的，而 pAgNPs 是凋亡的。JC-1 染料染色显示线粒体膜电位在 pAgNPs 处理时受到影响，而在 bAgNPs 处理时保持不变。流式细胞术证实了 HeLa 细胞在用纳米颗粒处理时细胞周期停滞，在 pAgNPs 的情况下进一步导致细胞凋亡。在用 bAgNPs 和 pAgNPs 处理后，分别发现约 77% 和 58% 的 HeLa 细胞处于亚 G1 期。bAgNPs 对 HeLa 细胞显示出细胞抑制作用，阻止细胞在亚 G1 期生长，而，
结论
总体而言，bAgNPs 和 pAgNPs 可能是安全的，并显示出用作抗人宫颈癌细胞的抗癌纳米抗生素的潜力。
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