Abstract Acanthoscelides obtectus is an insect pest feeding on common beans Phaseolus vulgaris. Three strains of Trichoderma harzianum and one strain of Trichoderma citrinoviride were sprayed on insect adults and Phaseolus vulgaris L. beans in order to investigate the efficiency of the fungi in combating Acanthoscelides obtectus insect pest. The strains E20, T34, and T019 exhibited high virulence on adults. Spores of T34 isolate were more attractant for both sexes and showed a higher mortality rate on insects in contact with treated beans than the other strains. Treated beans showed a lower weight loss compared to the untreated ones. T019 and T008 treatments resulted in a lower weight loss of beans than the other fungal strains. Daily insect emergence was reduced in treated beans. The strain T34 accounted for the lowest daily emergence of insects. T008, T019, and E20 exhibited the capacity to repel insects, which resulted in a reduction of damaged beans as well as a lower number of holes per bean. Trichoderma strains were re-isolated from insects’ cadavers extracted from treated beans. Regarding these results, T34 strain could be used in the control of A. obtectus adults due to its high lethal effect. Additionally, T008 and T019 strains, showing good results in terms of insecticidal activity, insect repellency, reduction of damaged beans and lower weight loss of beans, could also be used for the biological control of A. obtectus.

中文翻译：

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喷洒在 Acanthoscelides obtectus 和 Phaseolus vulgaris L. beans 上的木霉菌株孢子：对豆象鼻虫生物学的影响

摘要 Acanthoscelides obtectus 是一种以菜豆菜豆为食的害虫。将三株哈茨木霉和一株柑橘木霉喷洒在成虫和菜豆上，以研究真菌对抗 Acanthoscelides obtectus 害虫的效率。菌株 E20、T34 和 T019 对成人表现出高毒力。T34 分离株的孢子对两性都更具吸引力，并且与其他菌株相比，与处理过的豆类接触的昆虫的死亡率更高。与未经处理的豆类相比，经过处理的豆类显示出较低的重量损失。T019 和 T008 处理导致豆类的重量损失低于其他真菌菌株。处理过的豆类中每日昆虫出现减少。菌株 T34 占昆虫每日出现次数最低的原因。T008、T019 和 E20 表现出驱虫能力，这导致受损豆类减少以及每个豆类的孔数减少。从从处理过的豆类中提取的昆虫尸体中重新分离出木霉属菌株。关于这些结果，T34 菌株由于其高致死作用可用于控制 A. obtectus 成虫。此外，T008和T019菌株在杀虫活性、驱虫性、减少豆类损伤和降低豆类重量损失方面表现出良好的效果，也可用于A. obtectus的生物防治。关于这些结果，T34 菌株由于其高致死作用可用于控制 A. obtectus 成虫。此外，T008和T019菌株在杀虫活性、驱虫性、减少豆类损伤和降低豆类重量损失方面表现出良好的效果，也可用于A. obtectus的生物防治。关于这些结果，T34 菌株由于其高致死作用可用于控制 A. obtectus 成虫。此外，T008和T019菌株在杀虫活性、驱虫性、减少豆类损伤和降低豆类重量损失方面表现出良好的效果，也可用于A. obtectus的生物防治。