Carassius auratus gibelio is susceptible to the herpesviral hematopoietic necrosis (HVHN) disease caused by cyprinid herpesvirus 2 (CyHV-2) infection during the breeding process. Nevertheless, the report on biological response of CyHV-2 with C. auratus gibelio was limited, especially in vitro. In this study, host gene expression profiling was mostly analyzed in caudal fin cells of Carassius auratus gibelio (GiCF) underlying CyHV-2 infection. Transcriptomics and proteomics were employed to study the differential expression gene and revealed the host genes involved in pathway during the CyHV-2 infection. Transcriptome analysis revealed that compared with the control group, there were 11 335 and 19 421 differentially expressed unigenes at 48 h and at 96 h, respectively. Furthermore, proteome analysis showed that there were a total of 9008 proteins, among which 169 proteins were differential expression in the 48 h group and 502 proteins in the 96 h group. Notably, 10 and 158 differentially co-expressed genes at mRNA and protein levels (cDEGs) were reliably quantified at 48 h and 96 h, respectively. Interestingly, significantly different expressed genes both in the transcriptome and the proteome were identified, including GNG7, Hsp90a, THBS1 and RRM2. The result suggested that PI3k-AKT pathway was activated, but the p53 signaling pathway was suppressed. The above result will lay the foundation for understanding the mechanisms of host defense virus invasion during CyHV-2 infection.

assi鱼（Carassius auratus gibelio）在繁殖过程中易受塞浦路斯疱疹病毒2（CyHV-2）感染引起的疱疹病毒性造血坏死（HVHN）疾病的影响。尽管如此，与CyHV-2的生物反应的报告C.鲫是有限的，尤其是在体外。在这项研究中，主机基因表达谱进行分析大多在尾鳍细胞银鲫（GiCF）潜在的CyHV-2感染。转录组学和蛋白质组学被用于研究差异表达基因，并揭示了在CyHV-2感染过程中参与途径的宿主基因。转录组分析显示，与对照组相比，在48 h和96 h分别有11 335和19 421差异表达的单基因。蛋白质组分析结果表明，共有9008个蛋白质，其中在48 h组有169个差异表达蛋白，在96 h组有502个差异表达蛋白。值得注意的是，分别在48 h和96 h分别对mRNA和蛋白水平（cDEGs）上10和158个差异共表达的基因进行了可靠的定量。有趣的是，在转录组和蛋白质组中都发现了显着不同的表达基因，包括GNG7，Hsp90a，THBS1和RRM2。结果表明PI3k-AKT通路被激活，但p53信号通路被抑制。以上结果将为了解CyHV-2感染过程中宿主防御病毒入侵的机制奠定基础。