Microbial communities greatly affect rearing water quality and the larvae health during shrimp hatchery periods. In this study, we investigated the microbial communities of rearing water and larvae of Litopenaeus vannamei after treating hatchery water with different kinds of chemical disinfectants: no disinfectants (Con), chlorine dioxide (ClO₂), formaldehyde solution (HCHO), bleach powder (CaClO), and iodine (I₂). The water and larval samples were collected from nauplius 6 (N6), zoea 1 (Z1), mysis 1 (M1), and postlarvae 1 (P1) shrimp growth periods. 16S rDNA high-throughput sequencing revealed that the bacterial composition of the rearing water was more complex than that of the larvae, and the bacterial community of the rearing water and the larvae fluctuated significantly at the P1 and Z1 periods, respectively. Disinfectants altered the bacterial diversity and composition of the rearing water and larvae. Specifically, in the rearing water of the P1 period, Proteobacteria abundance was increased in the HCHO group; while Bacteroidetes abundance was decreased in the ClO₂, HCHO, and I₂ groups but increased in the CaClO group. In the larvae of the Z1 period, Firmicutes (especially Bacillus class) abundance was increased in the CaClO group, but decreased in the ClO₂, HCHO, and I₂ groups. Network analyses revealed that the genera Donghicola, Roseibacterium, Candidatus-Cquiluna, and Nautella were enriched in the rearing water, while Halomonas, Vibrio, and Flavirhabdus had high abundance in the larvae. The survival of shrimp was influenced by disinfectants that were inconsistent with the bacterial community changes. These results will be helpful for using microbial characteristics to facilitate healthy shrimp nursery.

中文翻译：

---

孵化场水消毒处理后不同生长阶段凡纳滨对虾幼虫和饲养水中微生物群落的变化。

在虾孵化期间，微生物群落极大地影响饲养水质和幼体健康。在这项研究中，我们调查了南美白对虾（Litopenaeus vannamei）的饲养水和幼虫在用不同类型的化学消毒剂处理孵化水后的微生物群落：无消毒剂（Con），二氧化氯（ClO ₂），甲醛溶液（HCHO），漂白粉（ CaClO）和碘（I ₂）。从无节幼体6（N6），zoea 1（Z1），mysis 1（M1）和幼虫1（P1）虾生长期收集水和幼体样品。16S rDNA高通量测序表明，饲养水的细菌组成比幼虫更复杂，并且饲养水和幼虫的细菌群落分别在P1和Z1时期显着波动。消毒剂改变了饲养水和幼虫的细菌多样性和组成。具体来说，在P1期的饲养水中，HCHO组的变形杆菌细菌丰度增加了。而在ClO ₂，HCHO和I _2中拟杆菌的丰度降低组，但CaClO组增加。在Z1期的幼虫中，CaClO组的纤毛虫（尤其是芽孢杆菌类）的丰度增加，而ClO ₂，HCHO和I ₂组的丰满菌减少。网络分析表明，东瑞科属，玫瑰杆菌属，念珠菌-克雷库纳属和鹦鹉螺属在饲养水中富集，而梭菌，弧菌和黄病毒属幼虫中的丰度较高。虾的生存受到与细菌群落变化不一致的消毒剂的影响。这些结果将有助于利用微生物特性促进健康的虾苗场。