Establishing empirical design rules of nucleic acid templates for the synthesis of silver nanoclusters with tunable photoluminescence and functionalities towards targeted bioimaging applications,Nanoscale Advances

当前位置： X-MOL 学术 › Nanoscale Adv. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Establishing empirical design rules of nucleic acid templates for the synthesis of silver nanoclusters with tunable photoluminescence and functionalities towards targeted bioimaging applications
Nanoscale Advances ( IF 4.6 ) Pub Date : 2020-07-23 , DOI: 10.1039/d0na00381f
Jason Y C Lim ₁ , Yong Yu ₁ , Guorui Jin ₁ , Kai Li ₁ , Yi Lu ₂ , Jianping Xie ₃ , Yen Nee Tan _{1,

4}

Affiliation

DNA-templated silver nanoclusters (AgNCs) are an emerging class of ultrasmall (<2 nm) fluorophores with increasing popularity for bioimaging due to their facile synthesis and tunable emission color. However, design rules correlating different nucleotide sequences with the photoemission properties of AgNCs are still largely unknown, preventing the rational design of DNA templates to fine-tune the emission color, brightness and functionalities of AgNCs for any targeted applications. Herein, we report a systematic investigation to understand the empirical influences of the four basic DNA nucleotides on AgNC synthesis and their effects on photoluminescence properties. After establishing the importance of nucleotide–Ag⁺ binding and AgNC encapsulation within DNA tetraplex structures, we then determined the unique attributes of each individual nucleobase using different combinations of systematically varied DNA templates. Using the empirical design rules established herein, we were able to predict the photoluminescence behaviours of AgNCs templated by complex aptamer sequences with specific binding affinity to human cancer cells, and to deliberately control their emission color by rational modifications of the DNA template sequences for targeted bioimaging. Our empirical findings from this systematic experimentation can contribute towards the rational design of DNA sequences to customise the photoluminescence properties and biofunctionalities of DNA-protected AgNCs towards multicolour targeted bioimaging applications.

中文翻译：

建立核酸模板的经验设计规则，用于合成具有可调光致发光和靶向生物成像应用功能的银纳米团簇

DNA 模板银纳米团簇 (AgNC) 是一类新兴的超小 (<2 nm) 荧光团，由于其易于合成和可调的发射颜色，在生物成像中越来越受欢迎。然而，将不同核苷酸序列与 AgNC 的光电发射特性相关联的设计规则仍然很大程度上未知，这阻碍了 DNA 模板的合理设计，以针对任何目标应用微调 AgNC 的发射颜色、亮度和功能。在此，我们报告了一项系统研究，以了解四种基本 DNA 核苷酸对 AgNC 合成的经验影响及其对光致发光特性的影响。在确定了 DNA 四链体结构中核苷酸-Ag ⁺结合和 AgNC 封装的重要性后，我们使用系统变化的 DNA 模板的不同组合确定了每个核碱基的独特属性。利用本文建立的经验设计规则，我们能够预测以与人类癌细胞具有特异性结合亲和力的复杂适体序列为模板的AgNC的光致发光行为，并通过合理修饰DNA模板序列来有意控制其发射颜色以进行靶向生物成像。我们从这个系统实验中得到的经验结果可以有助于 DNA 序列的合理设计，以定制 DNA 保护的 AgNC 的光致发光特性和生物功能，以实现多色靶向生物成像应用。

更新日期：2020-09-16

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文