ABSTRACT

In the present study, the effects of arbuscular mycorrhizal fungi, Funneliformis mosseae and Rhizophagus irregularis, on oxygen radical scavenging system (superoxide dismutase, catalase and peroxidase), osmotic adjustments (proline and soluble carbohydrates), relative water content, root colonization, leaf nutrients (N, P, K) and biological yield of Melilotus officinalis under rainfed and supplemental irrigation were studied in the morning and the afternoon light conditions in agrisilviculture system. A 2-year split-plot experiment was carried out using randomized complete block design with three replications in a plum (Prunus domestica (L.), Opal) orchard. Antioxidant activity was stimulated more in no-mycorrhizal inoculated plants in both of the irrigation systems for both of the light conditions. Enzymatic and non-enzymatic antioxidants were decreased in mycorrhizal stressed plants. Despite the increase in the leaf total chlorophyll in mycorrhized plants, the concentrations of carotenoid were decreased. In irrigated plants, significant increases of biological yield and leaf nutrients were observed in the morning light condition for mycorrhization, similar to the rainfed. Inoculation with F. mosseae generated more biological yield in the morning light area. Water status improvement through declined antioxidant activity and osmolytes accumulation in mycorrhized plants resulted in compensation for yield loss.

Abbreviations: AMF: arbuscular mycorrhizal fungi; CAT: catalase; Funneliformis mosseaeL F. mosseae, H2O2: hydrogen peroxide; MDA: malondialdehyde; POX: peroxidase; ROS: reactive oxygen species; Rhizophagus irregularis, R. irregularis; RWC: relative water content; SOD: superoxide dismutase; TSS: Total soluble sugar.

摘要

在本研究中，丛枝菌根真菌、Funneliformis mosseae和Rhizophagus Independentis对氧自由基清除系统（超氧化物歧化酶、过氧化氢酶和过氧化物酶）、渗透调节（脯氨酸和可溶性碳水化合物）、相对含水量、根系定植、叶片养分的影响（N，P，K）和生物产量黄香草木樨下旱地和补充灌溉在上午和下午在agrisilviculture系统光条件进行了研究。使用随机完全区组设计在李子 ( Prunusdomesica(L.), Opal) 果园。在两种光照条件下，两种灌溉系统中的无菌根接种植物的抗氧化活性受到更多刺激。在菌根胁迫的植物中，酶促和非酶促抗氧化剂减少。尽管菌根植物的叶总叶绿素增加，但类胡萝卜素的浓度却降低了。在灌溉植物中，在晨光条件下观察到菌根形成的生物产量和叶片养分显着增加，类似于雨育。接种F. mosseae在晨光区产生更多的生物产量。通过菌根化植物中抗氧化活性下降和渗透物积累来改善水分状况，从而补偿产量损失。

缩写： AMF：丛枝菌根真菌；CAT：过氧化氢酶；Funneliformis mosseaeL F. mosseae，H2O2：过氧化氢；MDA：丙二醛；POX：过氧化物酶；ROS：活性氧；不规则根瘤菌，不规则根瘤菌；RWC：相对含水量；SOD：超氧化物歧化酶；TSS：总可溶性糖。