Abstract

The study was conducted to determine anti-tyrosinase and antioxidant activities of the extracted collagen hydrolysate (CH) derived from Malaysian jellyfish, Rhopilema hispidum. Collagen was extracted using 1:1 (w:v) 0.1 M NaOH solution at temperature 25 °C for 48 hr followed by treatment of 1:2 (w:v) distilled water for another 24 hr and freeze-dried. The extracted collagen was hydrolyzed using papain at optimum temperature, pH and enzyme/substrate ratio [E/S] of 60 °C, 7.0 and 1:50, respectively. CH was found to exhibit tyrosinase inhibitory activity, DPPH radical scavenging and metal ion-chelating assays up to 64, 28, and 83%, respectively, after 8 hr of hydrolysis process. The molecular weight of CH was found <10 kDa consisting of mainly Gly (19.219%), Glu (10.428%), and Arg (8.848%). The UV–visible spectrum analysis showed a major and minor peak at 218 and 276 nm, accordingly. The FTIR spectroscopy confirmed the amide groups in CH. The SEM images demonstrated spongy and porous structure of CH. In the cytotoxicity study, CH has no cytotoxicity against mouse embryonic 3T3 fibroblast cell line with IC₅₀ value >500 µg/ml. Results revealed that the CH generated from this study has a potential to be developed as active ingredient in cosmeceutical application.

摘要

进行该研究以确定提取自马来西亚水母Rhopilema hispidum的提取的胶原蛋白水解产物（CH）的抗酪氨酸酶和抗氧化活性。在25°C的温度下，使用1：1（w：v）0.1 M NaOH溶液提取胶原蛋白48小时，然后将1：2（w：v）蒸馏水再处理24小时，然后冷冻干燥。使用木瓜蛋白酶在最佳温度，pH和酶/底物比[E / S]分别为60°C，7.0和1:50的条件下水解提取的胶原蛋白。在水解过程8小时后，发现CH表现出酪氨酸酶抑制活性，DPPH自由基清除和金属离子螯合试验，分别高达64％，28％和83％。发现CH的分子量＜10kDa，主要由Gly（19.219％），Glu（10.428％）和Arg（8.848％）组成。紫外可见光谱分析显示出在218和276 nm处的主要和次要峰。FTIR光谱证实了CH中的酰胺基。SEM图像显示了CH的海绵状和多孔结构。₅₀值> 500 µg / ml。结果显示，从这项研究中产生的CH有潜力被开发为药妆应用中的活性成分。