ABSTRACT Highly polyphagous rugose spiralling whitefly (RSW), Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae) invaded India during 2016. Pathogenicity of two strains (ICAR-NBAIR Pfu-1 & Pfu-5) of entomopathogenic fungus Isaria fumosorosea Wize were evaluated against RSW, however, only Pfu-5 showed virulence. Further dose mortality studies were carried out with re-isolated Pfu-5 strain with five different concentrations ranging from 1×104–1 × 108 spores/mL on A. rugioperculatus and its dominant parasitoid Encarsia guadeloupae (Hymenoptera: Aphelinidae). The Pfu-5 strain showed mean mortality of 44.03, 44.80, 36.42 and 28.82% on the eggs, first, third and fourth instar nymphs, respectively. The LC50 values of I. fumosorosea for eggs, first, third and fourth instar nymphs were 2.6 × 104, 2.3 × 104, 3.5 × 105 and 9.5 × 105 spores/mL, respectively, and the LT50 values were 50.39, 41.39, 67.54 and 68.31 h, respectively, for corresponding life stages for the concentration of 1 × 108 spores/mL. The impacts of I. fumosorosea on the parasitoid showed the inhibition of adult emergence was much lower in the Pfu-5 strain (5.8–19.6%) at a concentration of 1 × 108 spores/mL at different exposure times. The present study indicated the pathogenicity of Pfu-5 against A. rugioperculatus and warrant further studies on the compatibility of these biocontrol agents to be evaluated for the simultaneous utilisation under field conditions.

中文翻译：

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昆虫病原真菌Isaria fumosorosea对皱纹螺旋粉虱Aleurodicus rugioperculatus的致病性及其对寄生蜂Encarsia guadeloupae的影响

摘要 高度多食皱纹螺旋粉虱 (RSW)，Aleurodicus rugioperculatus Martin（半翅目：粉虱科）于 2016 年入侵印度。对昆虫病原真菌 Isaria fumosorose 的两种菌株（ICAR-NBAIR Pfu-1 和 Pfu-5）的致病性进行了评估然而，只有 Pfu-5 显示出毒力。对重新分离的 Pfu-5 菌株进行了进一步的剂量死亡率研究，对 A. rugioperculatus 及其优势寄生蜂 Encarsia guadeloupae（膜翅目：Aphelinidae）上的五种不同浓度范围为 1×104–1×108 孢子/mL 不等。Pfu-5 菌株对一、三、四龄若虫卵的平均死亡率分别为 44.03、44.80、36.42 和 28.82%。I. fumosorosea 对一、三、四龄若虫卵的LC50值为2.6×104、2.3×104、3.5×105和9.5×105孢子/mL，分别为 50.39、41.39、67.54 和 68.31 h，对于 1 × 108 孢子/mL 浓度的相应生命阶段的 LT50 值分别为。I. fumosorosea 对寄生蜂的影响表明，在不同暴露时间，浓度为 1 × 108 孢子/mL 的 Pfu-5 菌株（5.8-19.6%）对成虫羽化的抑制要低得多。本研究表明 Pfu-5 对 A. rugioperculatus 的致病性，并需要进一步研究这些生物防治剂的相容性，以评估在田间条件下同时使用的情况。对寄生物的 fumosorosea 显示，在不同暴露时间，浓度为 1 × 108 孢子/mL 的 Pfu-5 菌株（5.8-19.6%）对成虫羽化的抑制要低得多。本研究表明 Pfu-5 对 A. rugioperculatus 的致病性，并需要进一步研究这些生物防治剂的相容性，以评估在田间条件下同时使用的情况。对寄生物的 fumosorosea 显示，在不同暴露时间，浓度为 1 × 108 孢子/mL 的 Pfu-5 菌株（5.8-19.6%）对成虫羽化的抑制要低得多。本研究表明 Pfu-5 对 A. rugioperculatus 的致病性，并需要进一步研究这些生物防治剂的相容性，以评估在田间条件下同时使用的情况。