Abstract

In this study, new polymers containing amides (TrisPS-Ntaa, and TrisPS-Ntaa-Fc) were synthesized by condensation reaction for qualitative identification of insecticides. The synthesized polymers, including amides were investigated by infrared spectroscopy (IR), scanning electron microscopy-energy dispersion X- ray spectrometry (SEM-EDX), and gel permeation chromatography (GPC). Then, acetylcholinesterase enzyme (AChE) was covalently immobilized on these polymers to improve properties (including activity, reusability, and storage stability). Accordingly, organophosphate (malathion, acephate, chlorpyrifos methyl) and carbamate (carbofuran, methiocarb, methomyl), which are used to prevent harmful organisms in some agricultural products were enzymatically determined based on their inhibitory activity on AChE.

摘要

在这项研究中，含有酰胺的新型聚合物（TrisPS-Ntaa和TrisPS-Ntaa-Fc通过缩合反应合成）以定性鉴定杀虫剂。通过红外光谱（IR），扫描电子显微镜-能量分散X射线光谱法（SEM-EDX）和凝胶渗透色谱法（GPC）研究了包括酰胺的合成聚合物。然后，将乙酰胆碱酯酶（AChE）共价固定在这些聚合物上，以改善性能（包括活性，可重复使用性和储存稳定性）。因此，根据酶对AChE的抑制活性，通过酶法确定了用于预防某些农产品中有害生物的有机磷酸酯（马拉硫磷，乙酰甲胺磷，甲基毒死rif）和氨基甲酸酯（碳呋喃，甲硫威，灭多威）。