Propamocarb hydrochloride is a systemic fungicide used against Pythium and Phytophthora as well as other diseases in tobacco. This study aimed to understand the response of tobacco to propamocarb hydrochloride treatment at the transcriptome and metabolome levels. Transcriptomic and metabolomic analyses were conducted on tobacco leaves treated with or without propamocarb hydrochloride to identify differentially expressed genes (DEGs) and differential metabolites, which were used for further functional enrichment analyses. In all, 1364 to 2497 DEGs and 34 differential metabolites were identified in different comparable groups. The DEGs were significantly involved in functions related to photosynthesis and valine, leucine, and isoleucine degradation. Differential metabolites were mainly associated with arginine and proline metabolism (1,3‐diaminopropane; 4‐aminobutyric acid; and N‐(omega)‐hydroxyarginine) and glycerophospholipid metabolism (lysoPC(16:0), phosphorylcholine, and triethanolamine). Our study identified differential genes and metabolites that were associated with the response of tobacco leaves to propamocarb hydrochloride. Propamocarb hydrochloride could influence the photosynthesis, amino acid metabolism, and glycerophospholipid metabolism of tobacco leaves.

中文翻译：

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烟草中盐酸丙氨威酯反应相关基因和代谢产物的分析

盐酸丙溴威是一种用于对抗腐霉菌和疫霉菌的内用杀菌剂以及烟草中的其他疾病。这项研究的目的是了解烟草在转录组和代谢组水平上对盐酸丙氨威酯处理的反应。对经过或不经过盐酸丙莫卡威处理的烟叶进行转录组和代谢组学分析，以鉴定差异表达基因（DEG）和差异代谢物，将其用于进一步的功能富集分析。在不同的可比较组中总共鉴定出了1364至2497个DEG和34种不同的代谢产物。DEGs显着参与与光合作用和缬氨酸，亮氨酸和异亮氨酸降解有关的功能。差异代谢物主要与精氨酸和脯氨酸代谢有关（1,3-二氨基丙烷； 4-氨基丁酸；N-（ω）-羟基精氨酸）和甘油磷脂代谢（lysoPC（16：0），磷酸胆碱和三乙醇胺）。我们的研究确定了与烟叶对盐酸丙氨威的反应相关的差异基因和代谢产物。盐酸丙溴威可能影响烟叶的光合作用，氨基酸代谢和甘油磷脂代谢。