This work describes the first confirmed cases of carp oedema virus disease (CEVD) in Slovakia and the Czech Republic and the phylogenetic analysis of Czech and Slovak carp oedema virus (CEV) isolates. Four cases of disease outbreak in the Czech Republic are described, the oldest dating from mid‐May 2013 and one case from Slovakia dating from May 2019. In all cases, virus presence was confirmed using nested PCR. PCR products were sequenced and compared with 357‐bp nucleotide sequences encoding the CEV P4a protein in GenBank. In four cases of disease outbreak (three common carp breeding facilities and one koi garden pond), CEV detected belonged to genogroup I. In one case (koi garden pond), fish were confirmed as infected with CEV from genogroup II. This work complements data on CEV occurrence in European countries and contributes to a better understanding of the pathways leading to transmission of the virus throughout Europe.

中文翻译：

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在捷克和斯洛伐克的水产养殖中爆发鲤鱼水肿病毒病。

这项工作描述了斯洛伐克和捷克共和国首例确诊的鲤鱼浮肿病毒病（CEVD）病例，以及捷克和斯洛伐克鲤鱼浮肿病毒（CEV）分离株的系统发育分析。描述了捷克共和国的四起疾病暴发，最早的一次是2013年5月中旬，另一起的斯洛伐克是2019年5月。在所有情况下，都使用巢式PCR确认了病毒的存在。对PCR产物进行测序，并与GenBank中编码CEV P4a蛋白的357-bp核苷酸序列进行比较。在四例疾病暴发（三个鲤鱼繁育设施和一个锦鲤花园池塘）中，检测到的CEV属于基因组I。在一个案例（锦鲤花园池塘）中，已确认鱼类感染了基因组II的CEV。