Effects of co-administration of rapamycin and evening primrose/hemp seed oil supplement on immunologic factors and cell membrane fatty acids in experimental autoimmune encephalomyelitis.,Gene

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Effects of co-administration of rapamycin and evening primrose/hemp seed oil supplement on immunologic factors and cell membrane fatty acids in experimental autoimmune encephalomyelitis.
Gene ( IF 2.6 ) Pub Date : 2020-07-23 , DOI: 10.1016/j.gene.2020.144987
Soheila Rezapour-Firouzi ₁ , Mahshid Mohammadian ₂ , Maryam Sadeghzadeh ₃ , Ebrahim Mazloomi ₁

Affiliation

Background

The immune response is influenced by the administration of omega-3 polyunsaturated fatty acids (PUFA). Multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE) are affected by PUFA. The combination of evening primrose/hemp seed oil (EPO / HSO) has essential fatty acids (EFAs) for human optimal health due to the favorable ratio of omega-6 / omega-3 and antioxidant properties. The study was conducted to evaluate the effects of EPO / HSO on improving the composition of membrane fatty acids on spleen and blood cells and immunologic factors compared to rapamycin (RAPA) in the EAE model.

Methods and materials

Chronic-EAE was induced by induction of MOG in C57BL/6J mice (female, age: 6-8 weeks, weight 18-21). Mice were assigned to 5 groups (6 / group) to evaluate the therapeutic effects of EPO / HSO supplement in comparison with rapamycin: A group; EPO / HSO + RAPA, B group; RAPA, C group; EPO / HSO. Results were compared to two control groups (EAE and naive). The fatty acid profile of the spleen and blood cell membrane was evaluated. Real-time-polymerase chain reaction (RT-PCR) was used for the purpose of gene expression interleukin (IL) -4, IL-5, and IL-13 of lymphocytes. Also, IL-4 of serum was evaluated by enzyme-linked immunosorbent assay (ELISA).

Results

Our findings indicated that EPO / HSO therapy significantly increased the percentage of essential fatty acids in cell membranes of the spleen and blood. The relative expression of IL-4, IL-5, and IL-13 genes and serum levels of IL-4 were significantly increased in the HSO / EPO treated group.

Conclusion

These results point to potential therapeutic effects on the repair of the structure of cell membranes by EPO / HSO in EAE.

中文翻译：

雷帕霉素和月见草/麻籽油补充剂共同给药对实验性自身免疫性脑脊髓炎的免疫学因子和细胞膜脂肪酸的影响。

背景

免疫反应受omega-3多不饱和脂肪酸（PUFA）施用的影响。多发性硬化症（MS）及其动物模型，实验性自身免疫性脑脊髓炎（EAE）受PUFA影响。月见草/大麻子油（EPO / HSO）的组合具有人体必需的必需脂肪酸（EFAs），因为它们具有有利的omega-6 / omega-3比例和抗氧化性能。进行该研究以评估EPO模型中与雷帕霉素（RAPA）相比，EPO / HSO对改善脾脏和血细胞膜脂肪酸组成以及免疫因子的影响。

方法和材料

在C57BL / 6J小鼠（雌性，年龄：6-8周，体重18-21）中，通过MOG诱导诱导了慢性EAE。将小鼠分为5组（6组/组），以评估EPO / HSO补充剂与雷帕霉素的治疗效果。EPO / HSO + RAPA，B组；RAPA，C组；EPO / HSO。将结果与两个对照组（EAE和天真）进行比较。评估脾脏和血细胞膜的脂肪酸谱。实时聚合酶链反应（RT-PCR）用于淋巴细胞基因表达白介素（IL）-4，IL-5和IL-13的目的。另外，通过酶联免疫吸附测定（ELISA）评价血清的IL-4。