Green mould disease of mushroom, Agaricus bisporus,is caused by Trichodermaspecies and can result in substantial crop losses.Label free proteomic analysis of changes in the abundance of A. bisporusproteins following exposure to T. aggressivumsupernatantin vitroindicated increased abundance of proteins associated with an oxidative stress response (zinc ion binding (+6.6 fold); peroxidase activity (5.3-fold); carboxylic ester hydrolase (+2.4 fold); dipeptidase (+3.2 fold); [2Fe-2S] cluster assembly (+3.3 fold)). Proteins that decreased in relative abundance were associated with growth: structural constituent of ribosome, translation (-12 fold), deadenylation-dependent decapping of nuclear-transcribed mRNA (-3.4 fold), and small GTPase mediated signal transduction (-2.6 fold). In vivoanalysis revealed that 10-4 T. aggressivuminoculum decreased the mushroom yield by 29% to 56% and 10-3 T. aggressivuminoculum decreased the mushroom yield by 68% to 100%. Proteins that increased in abundance in A. bisporusin vivofollowing exposure to T. aggressivumindicated an oxidative stress response and included proteins with pyruvate kinase activity (+2.6 fold) and hydrolase activity (+2.1 fold)). The results indicate that exposure of A. bisporusmycelium to T. aggressivum in vitroand in vivoresulted in an oxidative stress response and reduction in growth.

中文翻译：

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双孢蘑菇暴露于 Trichoderma aggressivum f. europaeum 导致生长抑制和氧化应激反应的诱导

蘑菇的绿霉病，双孢蘑菇，由木霉属物种引起，可导致大量作物损失。对暴露于 T. aggressivumsupernatantin 体外后双孢蘑菇蛋白丰度变化的无标记蛋白质组学分析表明，与氧化应激相关的蛋白质丰度增加响应（锌离子结合（+6.6 倍）；过氧化物酶活性（5.3 倍）；羧酸酯水解酶（+2.4 倍）；二肽酶（+3.2 倍）；[2Fe-2S] 簇组装（+3.3 倍））。相对丰度降低的蛋白质与生长有关：核糖体的结构成分、翻译（-12 倍）、核转录 mRNA 的去腺苷酸化依赖性脱帽（-3.4 倍）和小 GTPase 介导的信号转导（-2.6 倍）。体内分析显示，10-4 T. aggressivuminoculum 将蘑菇产量降低了 29% 至 56%，而 10-3 T. aggressivuminoculum 将蘑菇产量降低了 68% 至 100%。暴露于 T. aggressivum 后，双孢菌素体内丰度增加的蛋白质表明存在氧化应激反应，包括具有丙酮酸激酶活性（+2.6 倍）和水解酶活性（+2.1 倍）的蛋白质。结果表明，A.bisporusmycelium 在体外和体内暴露于 T.aggressivum 导致氧化应激反应和生长减少。6 倍）和水解酶活性（+2.1 倍））。结果表明，A.bisporusmycelium 在体外和体内暴露于 T.aggressivum 导致氧化应激反应和生长减少。6 倍）和水解酶活性（+2.1 倍））。结果表明，A.bisporusmycelium 在体外和体内暴露于 T.aggressivum 导致氧化应激反应和生长减少。