Myo-inositol is an essential vitamin for most animals, and it can modulate multiple physiological functions. In this study, we performed transcriptome gene expression profiling of gill tissue from turbot Scophthalmus maximus fed different concentrations of myo-inositol (0, 300, 600, 900, 1200 mg/kg). Results of expression tendency analysis, Weighted Gene Co-Expression Network Analysis (WGCNA), integrated transcriptome analyses, and KEGG annotation analysis of all differentially expressed genes (DEGs) demonstrated that the cytokine-cytokine receptor interaction played a core role in effects of myo-inositol on turbot, which was followed by the Jak-STAT signaling pathway. The results of qRT-PCR also showed myo-inositol mediated the gene expression of the cytokine-cytokine receptor interaction and the Jak-STAT signaling pathway in turbot. The ELISA assay indicated that myo-inositol affected the concentration change of interleukins (IL-2 and IL-10). Consequently, the interleukins associated with immune functions in the cytokine-cytokine receptor interaction played a core role in the effects of myo-inositol on turbot, which was followed by the Jak-STAT signaling pathway. Additionally, 10 hub genes associated with myo-inositol-traits were identified via WGCNA.

肌肌醇是大多数动物必需的维生素，它可以调节多种生理功能。在这项研究中，我们进行了转录基因表达从大菱鲆鳃组织的仿形鲆馈送不同浓度的肌醇肌醇（0，300，600，900，1200毫克/千克）。对所有差异表达基因（DEG）的表达趋势分析，加权基因共表达网络分析（WGCNA），整合的转录组分析和KEGG注释分析的结果表明，细胞因子与细胞因子受体的相互作用在肌细胞的作用中起核心作用-菱turbo上的肌醇，随后是Jak-STAT信号通路。qRT-PCR的结果也显示出肌-肌醇介导大菱t中细胞因子-细胞因子受体相互作用和Jak-STAT信号通路的基因表达。ELISA测定表明，肌醇肌醇影响白细胞介素（IL-2和IL-10）的浓度变化。因此，在细胞因子-细胞因子受体相互作用的免疫功能相关联的白介素中的作用发挥了核心作用肌醇菱肌醇，随后由所述的Jak-STAT信号传导途径。另外，通过WGCNA鉴定了与肌醇特性相关的10个中枢基因。