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Boron analysis and imaging of cells with 2-hr BPA exposure by using micro-proton particle-induced gamma-ray emission (PIGE).
Applied Radiation and Isotopes ( IF 1.6 ) Pub Date : 2020-07-23 , DOI: 10.1016/j.apradiso.2020.109334 Kei Nakai 1 , Keita Endo 2 , Fumiyo Yoshida 2 , Masashi Koka 3 , Naoto Yamada 3 , Takahiro Satoh 3 , Takao Tsurubuchi 2 , Akira Matsumura 2 , Yoshitaka Matsumoto 4 , Hideyuki Sakurai 4
中文翻译:
使用微质子粒子诱导伽马射线发射 (PIGE) 对暴露 2 小时 BPA 的细胞进行硼分析和成像。
更新日期:2020-07-23
Applied Radiation and Isotopes ( IF 1.6 ) Pub Date : 2020-07-23 , DOI: 10.1016/j.apradiso.2020.109334 Kei Nakai 1 , Keita Endo 2 , Fumiyo Yoshida 2 , Masashi Koka 3 , Naoto Yamada 3 , Takahiro Satoh 3 , Takao Tsurubuchi 2 , Akira Matsumura 2 , Yoshitaka Matsumoto 4 , Hideyuki Sakurai 4
Affiliation
Particle-induced gamma-ray emission (PIGE), which measures prompt gamma rays at 428 keV from 10B (p, p'γ) 7Be, was used to confirm the boron distribution within 2hr-BPA-exposed cells. Distribution images of potassium, phosphate, and boron and the whole spectrum showed the ratios of boron counts to total (%) as follows: control group: 1.35 ± 0.073%; 2hr boron exposure group: 2.33 ± 0.35%; and boron exposure/wash group: 1.58 ± 0.095%. Micro-beam PIXE/PIGE can be a promising tool for visualization of intracellular Boron.
中文翻译:
使用微质子粒子诱导伽马射线发射 (PIGE) 对暴露 2 小时 BPA 的细胞进行硼分析和成像。
粒子诱导伽马射线发射 (PIGE) 可测量来自10 B (p, p'γ) 7 Be 的428 keV 瞬发伽马射线,用于确认 2 小时 BPA 暴露细胞内的硼分布。钾、磷、硼分布图及全谱显示硼计数占总量的比值(%)如下:对照组:1.35±0.073%;2hr硼暴露组:2.33±0.35%;和硼暴露/洗涤组:1.58 ± 0.095%。微束 PIXE/PIGE 可以成为细胞内硼可视化的有前途的工具。