Cutibacterium acnes (formerly Propionibacterium acnes) is the main bacterium targeted for the prevention and medical treatment of acne vulgaris. Lactic acid bacteria (LAB) are a group of microorganisms classified by their ability to produce lactic acid through fermentation. Although the activities of LAB have been studied, their potential anti-acne effects are not well known. Here, Lactobacillus paraplantarum THG-G10, which has anti-bacterial activity against C. acnes, was isolated from traditional Kimchi in Republic of Korea. The anti-acne effects of dried cell-free supernatant of L. paraplantarum THG-G10 (DC-G10) were evaluated by determining its anti-microbial and anti-inflammatory activities. Anti-microbial activity was examined by a broth dilution assay: 25 mg/ml of DC-G10 inhibited the growth of C. acnes KCTC 5012 and KACC 1194; salicylic acid and benzoyl peroxide for acne treatment inhibited the growth of C. acnes KCTC 5012 and KACC 11946 at concentrations of 1.25 and 7.5 mg/ml, respectively; and tea tree oil inhibited the growth of C. acnes KCTC 5012 but not the growth of C. acnes KACC 11946 at 50 mg/ml. Anti-inflammatory activity was evaluated by a nitric oxide (NO) assay: only DC-G10 and ascorbic acid reduced LPS-induced NO production in RAW 264.7 cells in a dose-dependent manner. In addition, the toxicities of erythromycin, salicylic acid, benzoyl peroxide, tea tree oil, and DC-G10 were examined in HaCaT cells and normal human dermal fibroblasts (NHDFs). In these cells, the cytotoxic effects of DC-G10 were weaker than the effects of erythromycin, benzoyl peroxide, and ascorbic acid. Furthermore, scanning electron microscopy revealed that DC-G10 induces deleterious morphological changes in the bacterial cell membrane. These results demonstrate that DC-G10 may be an effective and safe treatment for acne vulgaris.

痤疮皮肤杆菌（以前称为痤疮丙酸杆菌）是针对寻常型痤疮的预防和治疗的主要细菌。乳酸菌（LAB）是一组微生物，它们通过发酵产生乳酸的能力进行分类。尽管已经研究了LAB的活性，但其潜在的抗痤疮作用尚不为人所知。在这里，从大韩民国的传统泡菜中分离出具有对痤疮丙酸杆菌具有抗菌活性的副植物乳杆菌THG-G10 。干燥的副植物乳杆菌无细胞上清液的抗痤疮作用THG-G10（DC-G10）通过确定其抗微生物和抗炎活性进行评估。通过肉汤稀释测定法检测抗微生物活性：25 mg / ml DC-G10抑制痤疮丙酸杆菌KCTC 5012和KACC 1194的生长；水杨酸和过氧化苯甲酰用于痤疮治疗分别以1.25和7.5 mg / ml的浓度抑制痤疮丙酸杆菌KCTC 5012和KACC 11946的生长；和茶树油抑制生长C.杆菌KCTC 5012，但没有增长C.痤疮KACC 11946，50 mg / ml。通过一氧化氮（NO）分析评估抗炎活性：只有DC-G10和抗坏血酸以剂量依赖的方式减少RAW 264.7细胞中LPS诱导的NO产生。另外，在HaCaT细胞和正常人皮肤成纤维细胞（NHDF）中检查了红霉素，水杨酸，过氧化苯甲酰，茶树油和DC-G10的毒性。在这些细胞中，DC-G10的细胞毒性作用比红霉素，过氧化苯甲酰和抗坏血酸的作用弱。此外，扫描电子显微镜揭示DC-G10诱导细菌细胞膜中有害的形态变化。这些结果表明，DC-G10可能是一种有效而安全的寻常痤疮治疗方法。