In the present study, the influence of electrospinning parameters on poly(lactic acid) (PLA) nanofiber production and antimicrobial attitude of drug blending were investigated. The PLA concentrations (5 to 12% wt/v) were determined with solvent ratio variations of a triple solvent system (chloroform (CHL), dimethylformamide (DMF) and tetrahydrofuran (THF)). Flow rates were varied from 0.5 to 1 mL/h, while voltages were changed from 10 to 15 kV, and 10 kV voltage and 0.5 mL/h flow rate were selected as optimum electrospinning conditions. According to morphological investigations via scanning electron microscopy, the average PLA fiber diameters varied from 303 to 405 nm, for 5 and 8% wt/v PLA concentrations, respectively. The drug (ceftriaxone disodium) was blended into these concentrations and electrospun. Drug addition reduced the fiber diameters, and at 8% wt/v drug blended PLA concentrations homogeneous fiber distribution was obtained. Additionally, the antimicrobial attitude of drug blended PLA nanofibers was analyzed by using agar disc diffusion method and antimicrobial activity against Escherichia coli, and Listeria monocytogenes were observed.

Graphical abstract

在本研究中，研究了电纺丝参数对聚乳酸（PLA）纳米纤维生产和药物混合的抗菌态度的影响。通过三重溶剂系统（氯仿（CHL），二甲基甲酰胺（DMF）和四氢呋喃（THF））的溶剂比率变化来确定PLA浓度（5至12％wt / v）。流速从0.5到1 mL / h变化，而电压从10到15 kV改变，并且选择10 kV电压和0.5 mL / h流速作为最佳静电纺丝条件。根据通过扫描电子显微镜进行的形态学研究，对于5和8 wt / v的PLA浓度，平均PLA纤维直径从303到405 nm不等。将药物（头孢曲松二钠）混合至这些浓度并进行静电纺丝。药物添加减少了纤维直径，在8％wt / v的药物混合PLA浓度下，纤维分布均匀。此外，采用琼脂圆盘扩散法分析了掺聚PLA纳米纤维的抗菌态度，并对抗菌活性进行了研究。观察到大肠杆菌和李斯特菌。

图形概要