Magnetic nanoparticles (MNPs) especially iron oxide (Fe₃O₄) NPs have quite extensively been used for in vivo delivery of biomolecules and drugs because of their high bioconjugation efficiency. In this study, Fe₃O₄ NPs and (3-Aminopropyl) triethoxysilane (APTS) coated Fe₃O₄ NPs were synthesized and their interaction with Calf thymus (Ct) DNA has been studied in order to understand their usage in biomedical applications. Hydrothermal method was used for the NPs synthesis. Characterization of NPs was done using techniques like UV-Visible spectroscopy, FTIR spectroscopy, FE-SEM, EDAX, Zeta Sizer and powder XRD. Further, interaction studies of NPs with Ct-DNA were investigated using various physicochemical techniques. In UV-Visible studies, hypochromicity with binding constant 3.2 × 10⁵ M⁻¹ was observed. Binding constants calculated using fluorescence studies were found to be k = 3.2 × 10⁴ M⁻¹, 2.9 × 10⁴ M⁻¹ at 293 and 323 K respectively. Results of UV-Visible and fluorescence studies were in correlation with other techniques like UV-T_M and CD. All studies suggested alteration in DNA conformation on interaction with surface engineered Fe₃O₄ NPs, stabilizing DNA-NPs conjugate via partial intercalation and electrostatic interactions. This study may facilitate our understanding regarding the physicochemical properties and DNA-binding ability of APTS-Fe₃O₄ NPs for their further application in magnetosensitive biosensing and drug delivery.

Iron oxide based magnetic nanoparticles are well known for their excellent bio-conjugation efficiency and therefore APTS-Fe₃O₄ NPs were synthesized via very simple and benign hydrothermal method. Further, the interaction of APTS-Fe₃O₄ NPs with calf thymus DNA was studied using various physicochemical techniques to explore their potential in biomedical applications.

磁性纳米粒子（MNPs），尤其是氧化铁（Fe ₃ O ₄）NPs具有很高的生物结合效率，因此已被广泛用于体内生物分子和药物的递送。在这项研究中，Fe ₃ O ₄ NP和（3-氨基丙基）三乙氧基硅烷（APTS）涂覆的Fe ₃ O ₄合成了NP，并研究了它们与小牛胸腺（Ct）DNA的相互作用，以了解其在生物医学应用中的用途。水热法用于NP的合成。NPs的表征使用诸如紫外可见光谱，FTIR光谱，FE-SEM，EDAX，Zeta Sizer和粉末XRD等技术进行。此外，使用各种物理化学技术研究了NP与Ct-DNA的相互作用。在紫外线可见研究中，观察到结合常数为3.2×10 ⁵  M ^-1的色差降低。结合使用荧光研究计算常数被发现是K = 3.2×10 ⁴ 中号^-1，2.9×10 ⁴ 中号^-1分别位于293和323K。紫外可见光和荧光的研究结果与紫外像其他技术相关ŧ_中号和CD。所有研究都表明与表面工程化的Fe ₃ O ₄ NPs相互作用时DNA构象发生改变，通过部分插入和静电相互作用稳定DNA-NPs共轭物。这项研究可能有助于我们进一步了解APTS-Fe ₃ O ₄ NP的理化性质和DNA结合能力，以便进一步将其应用于磁敏生物传感和药物递送。

基于氧化铁的磁性纳米粒子以其出色的生物共轭效率而闻名，因此，APTS-Fe ₃ O ₄ NPs通过非常简单且良性的水热法合成。此外，使用各种物理化学技术研究了APTS-Fe ₃ O ₄ NP与小牛胸腺DNA的相互作用，以探索其在生物医学应用中的潜力。