Constructing a biomimetic scaffold that replicates the complex architecture of intervertebral disc annulus fibrosus (AF) remains a major goal in AF tissue engineering. In this study, a biomimetic angle-ply multi-lamellar polycaprolactone/silk fibroin (PCL/SF) AF scaffold was fabricated. Wet-spinning was used to obtain aligned PCL/SF microfiber sheets, and these were excised into strips with microfibers aligned at +30° or −30° relative to the strip long axis. This was followed by stacking two strips with opposing fiber alignment and wrapping them concentrically around a mandrel. Our results demonstrated that the scaffold possessed spatial structure and mechanical properties comparable to natural AF. The scaffold supported rabbit AF cells adhesion, proliferation, infiltration and guided oriented growth and extracellular matrix deposition. In conclusion, our angle-ply multi-lamellar scaffold offers a potential solution for AF replacement therapy and warrants further attention in future investigations.

构建仿生支架以复制椎间盘纤维环（AF）的复杂结构仍然是AF组织工程的主要目标。在这项研究中，仿生角多层多层聚己内酯/丝素蛋白（PCL / SF）AF支架。使用湿纺获得对准的PCL / SF超细纤维片，并将它们切成条状，其超细纤维相对于条带长轴以+ 30°或-30°对准。随后，将两根纤维排列相反的条带堆叠在一起，然后将它们同心地缠绕在心轴上。我们的结果表明，该支架具有与天然AF相当的空间结构和力学性能。该支架支持兔房颤细胞的粘附，增殖，浸润并指导定向生长和细胞外基质沉积。