Membrane material, pore size, and cleaning strategy are important factors for microalgal membrane harvesting. In this study, harvesting of Scenedesmus acuminatus cultivated in pilot scale using nine ultrafiltration and three microfiltration membranes was carried out in cross-flow filtration system to compare their filtration performance and flux recovery after physical and chemical cleaning. The 0.45-μm PVDF-AsahiKASEI membrane had the highest average flux of 513.6 L m⁻² h⁻¹ among 12 membranes and 50-kDa PVC-Litree membrane had the highest average flux of 98.0 L m⁻² h⁻¹ among nine ultrafiltration membranes for 60 L S. acuminatus suspension harvesting. There were significantly positive correlations between membrane pore size, pure water flux, and average flux for S. acuminatus suspension harvesting. Generally, ultrafiltration membrane had moderate total fouling index (TFI) and high proportion of hydraulic reversible fouling index (HRFI) of more than 95%. The 0.45-μm PVDF-AsahiKASEI membrane had a low TFI but the lowest proportion of HRFI, associated with the residual microalgal cells, debris, and colloids on the inner surface and top ends of some cells inserted to big membrane pores, demonstrated by SEM image and reduced porosity from 65.9 to 60.2%. Compared to soaking cleaning, circulation cleaning strategy had a better flux recovery efficiency for 0.45-μm PVDF-AsahiKASEI membrane, proving that it would be a potential way in membrane chemical cleaning. This study demonstrated that microfiltration with high flux has great potential in microalgal harvesting if flux recovery efficiency can be significantly increased using suitable cleaning strategy such as circulation cleaning strategy.

膜材料，孔径和清洁策略是微藻膜收获的重要因素。在这项研究中，在横流过滤系统中收获了使用九种超滤和三层微滤膜在中试规模下种植的景象，以比较它们在物理和化学清洗后的过滤性能和通量回收率。0.45μm的PVDF-AsahiKASEI膜在12个膜中的平均通量最高，为513.6 L m ^-2  h ^-1，而50 kDa PVC-Litree膜在9个超滤中的平均通量最高，为98.0 L m ^-2  h ^-1 60 L尖锐葡萄球菌膜悬浮收获。尖孔链霉菌的膜孔径，纯水通量和平均通量之间存在显着的正相关。悬浮收获。通常，超滤膜具有中等的总结垢指数（TFI）和高比例的水力可逆结垢指数（HRFI）超过95％。0.45-μm的PVDF-AsahiKASEI膜的TFI低，但HRFI的比例最低，这与插入大膜孔的一些细胞的内表面和顶端的残留微藻细胞，碎片和胶体有关，这是通过SEM图像证实的孔隙率从65.9％降低到60.2％。与浸泡清洗相比，循环清洗策略对0.45-μmPVDF-AsahiKASEI膜的通量回收效率更高，证明这是膜化学清洗的一种潜在方法。