We have previously isolated Bacillus subtilis HMNig-2 and MENO2 strains, from honey and the honeybee gut microbiome respectively, and demonstrated these strains to produce levansucrase with potential probiotics characteristics. Here we report their complete genome sequence and comparative analysis with other and other B. subtilis strains. The complete genome sequences of Bacillus subtilis HMNig-2 and MENO2 were de novo assembled from MiSeq paired-end sequence reads and annotated using the RAST tool. Whole-genome alignments were performed to identify functional differences associated with their potential use as probiotics. The comparative analysis and the availability of the genome sequence of these two strains will provide comprehensive analysis about the diversity of these valuable Bacillus strains and the possible impact of the environment on bacterial evolution. We introduce complete genome sequence of two new B. subtilis strains HMNig-2 and MENO2 with probiotic potential and as cell factories for the production of levan and other valuable components for pharmaceutical and industrial applications

中文翻译：

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从蜂蜜和蜜蜂微生物组中分离出的两种潜在益生菌枯草芽孢杆菌的完整基因组序列和比较分析。


我们之前分别从蜂蜜和蜜蜂肠道微生物组中分离出枯草芽孢杆菌 HMNig-2 和 MENO2 菌株，并证明这些菌株能够产生具有潜在益生菌特性的果聚糖蔗糖酶。在这里，我们报告了它们的完整基因组序列以及与其他枯草芽孢杆菌菌株的比较分析。枯草芽孢杆菌 HMNig-2 和 MENO2 的完整基因组序列是从 MiSeq 双端序列读取中重新组装的，并使用 RAST 工具进行注释。进行全基因组比对，以确定与其作为益生菌的潜在用途相关的功能差异。这两个菌株基因组序列的比较分析和可用性将为这些有价值的芽孢杆菌菌株的多样性以及环境对细菌进化可能的影响提供全面的分析。我们引入了两种新的枯草芽孢杆菌菌株 HMNig-2 和 MENO2 的完整基因组序列，它们具有益生菌潜力，可作为生产果聚糖和其他有价值的制药和工业应用成分的细胞工厂