In the present study, we evaluated new sources of plant proteases from fruits of Bromelia karatas (BK) and Bromelia pinguin (BP) to obtain antioxidant hydrolyzates/bioactive peptides (BPs) derived from chicken by-products (CH) and fish by-products (FH). The profile of the peptides was identified by reverse-phase high-resolution liquid chromatography (RP-HPLC) and the size weight distribution by molecular exclusion chromatography (SEC). The hydrolysates obtained with BK in both sources of by-products showed greater antioxidant capacity compared to those obtained with BP, presenting similar or higher values when compared to a commercial plant enzyme. The use of new sources of plant proteases allowed to obtain hydrolysates of hydrophilic character with a high percentage (> 50%) of peptides with molecular weights < 17.5 kDa from chicken and fish by-products. Therefore, based on the results obtained in antioxidant capacity it is possible to consider the hydrolysates as potential ingredients, food additives, and pharmaceutical products.

中文翻译：

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Bromelia karatas和Bromelia pinguin：用于从鸡肉和鱼类副产物中获得抗氧化剂水解产物的植物蛋白酶的来源

在本研究中，我们评估了来自凤梨（Bromelia karatas）和凤梨（Bromelia pinguin）果实（BP）的植物蛋白酶的新来源，以获得源自鸡副产品（CH）和鱼副产品的抗氧化剂水解物/生物活性肽（BPs）。 （FH）。通过反相高效液相色谱（RP-HPLC）鉴定肽的谱图，通过分子排阻色谱（SEC）鉴定大小分子量分布。与使用BP获得的水解产物相比，在两种副产物中用BK获得的水解产物均显示出更高的抗氧化能力，与商业植物酶相比具有相似或更高的值。使用新的植物蛋白酶来源可以得到亲水性的水解产物，并具有高百分比（> 50％）的分子量<17的肽。鸡肉和鱼类副产物为5 kDa。因此，基于抗氧化能力的结果，可以将水解产物视为潜在成分，食品添加剂和药品。