Wheat-derived arabinoxylans reduced M2-macrophage functional activity, but enhanced monocyte-recruitment capacity.,Food & Function

当前位置： X-MOL 学术 › Food Funct. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Wheat-derived arabinoxylans reduced M2-macrophage functional activity, but enhanced monocyte-recruitment capacity.
Food & Function ( IF 5.1 ) Pub Date : 2020-07-22 , DOI: 10.1039/d0fo00316f
Coen Govers ₁ , Yongfu Tang ₂ , Ellen H Stolte ₃ , Harry J Wichers ₄ , Jurriaan J Mes ₁

Affiliation

The immunomodulatory properties of non-digestible polysaccharides (NDPs) have been recognized in in vitro and in vivo studies. The latter mostly demonstrated altered frequencies and inflammatory status of immune cells as clinical parameters. Most of the NDP activity will be exerted in the intestine where they can directly interact with macrophages. The predominant macrophage phenotype in the intestine is M2-like, with M1-like macrophages arising during inflammation. Here, we investigated transcriptional and functional impact on these macrophage phenotypes by NDP-treatment (i.e. yeast-derived soluble β-glucan (yeast-βG), apple-derived RG-I (apple-RGI), shiitake-derived β-glucan (shiitake-βG) or wheat-derived arabinoxylan (wheat-AX)). Wheat-AX, and to a lesser extent shiitake-βG and apple-RGI but not yeast-βG, reduced endocytosis and antigen processing capacity of M1- and M2-like macrophages. Moreover, the NDPs, and most notably wheat-AX, strongly induced transcription and secretion of a unique set of cytokines and chemokines. Conditioned medium from wheat-AX-treated M2-like macrophages subsequently demonstrated strongly increased monocyte recruitment capacity. These findings are in line with clinically observed immunomodulatory aspects of NDPs making it tempting to speculate that clinical activity of some NDPs is mediated through enhanced chemoattraction and modifying activity of intestinal immune cells.

中文翻译：

小麦衍生的阿拉伯木聚糖降低了M2巨噬细胞的功能活性，但增强了单核细胞的招募能力。

在体外和体内研究中已经认识到非消化性多糖（NDP）的免疫调节特性。后者主要表现出改变的频率和免疫细胞的炎症状态作为临床参数。NDP的大部分活性将在肠道中发挥作用，它们可以直接与巨噬细胞相互作用。肠道中主要的巨噬细胞表型是M2样，在炎症过程中会出现M1样巨噬细胞。在这里，我们研究了NDP处理对这些巨噬细胞表型的转录和功能影响（即酵母来源的可溶性β-葡聚糖（酵母-βG），苹果来源的RG-I（apple-RGI），香菇来源的β-葡聚糖（shiitake-βG）或小麦来源的阿拉伯木聚糖（wheat-AX））。小麦-AX，以及较少量的香菇-βG和苹果-RGI，而不是酵母-βG，减少了M1和M2样巨噬细胞的内吞作用和抗原加工能力。此外，NDP，最著名的是小麦-AX，强烈诱导了一组独特的细胞因子和趋化因子的转录和分泌。来自小麦AX处理的M2样巨噬细胞的条件培养基随后证明单核细胞募集能力大大增强。这些发现与临床观察到的NDP的免疫调节特性是一致的，因此很容易推测某些NDP的临床活性是通过增强化学引诱力和修饰肠道免疫细胞的活性来介导的。

更新日期：2020-08-19

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11