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The Quality Control of Both Schisandra chinensis (Turcz.) Baill and Schisandra sphenanthera Rehd. Et Wils by Rapid Resolution Liquid Chromatography (RRLC)
Natural Product Communications ( IF 1.8 ) Pub Date : 2020-07-21 , DOI: 10.1177/1934578x20941278
Li Deng 1 , Chunping Yin 2 , Yuan-Chun Ma 3, 4
Affiliation  

Quality problems with Schisandra chinensis (Turcz.) Baill (SCB) and Schisandra sphenanthera Rehd.et Wils. (SSR) have been increasing owing to their obvious difference in supply and demand. In this study, qualitative and quantitative studies were carried out on the contents of various components of SCB and SSR, which provided the reference for the quality control of the raw materials of SCB and SSR. A ZORBAX Eclipse Plus C18 column (1.8 μm, 100 mm × 2.1 mm) was used with a column temperature of 40 °C, a mobile phase consisting of a gradient of acetonitrile and water, a flow rate of 0.3 mL/min, and an injection sample of 1 µL. Ultraviolet detection was operated at 220 nm throughout the analyzing time. Almost all the compounds achieved baseline separation. In addition, this method showed a favorable recovery (98.3%-102.5% for SCB and 99.1%-100.5% for SSR) and good precision and stability, confirming the reliability of the experimental data.



中文翻译:

北五味子五味子的质量控制。Et Wils的快速解析液相色谱(RRLC)

北五味子(Turcz。)Baill(SCB)和北五味子的生长质量问题Rehd.et Wils。(SSR)由于供需之间的明显差异而一直在增加。本研究对SCB和SSR各成分的含量进行了定性和定量研究,为SCB和SSR原料的质量控制提供了参考。使用ZORBAX Eclipse Plus C18色谱柱(1.8μm,100 mm×2.1 mm),色谱柱温度为40°C,流动相由乙腈和水的梯度组成,流速为0.3 mL / min,进样量为1 µL。在整个分析过程中,紫外线检测均在220 nm下进行。几乎所有化合物均达到了基线分离。此外,该方法显示出良好的回收率(SCB为98.3%-102.5%,SSR为99.1%-100.5%),并具有良好的精密度和稳定性,证实了实验数据的可靠性。

更新日期:2020-07-22
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