Abstract Three strains of fungi belonging to the genus Metarhizium Sorokīn (ARSEF 4556, ARSEF 3297, native strain) were assayed against adults and nymphs of the Neotropical brown stinkbug Euschistus heros (F.) and the green-belly stinkbug Dichelops furcatus (F.).The most virulent strain, ARSEF 4556, caused over 90% mortality. The average survival time of the second and fifth instar nymphs and adults following immersion in 1 × 108 conidia ml–1 was 4.8, 5.7, and 5.2 d, respectively. The second instar nymphs were more susceptible than the adults. The LC50 values and median survival times for second instar and adult E. heros were 1.6 × 107 and 3.1 × 107 conidia ml–1 and 6 and 8 d, respectively. Eggs of E. heros and the closely related stinkbug, D. furcatus, were highly susceptible to ARSEF 4556 with the mean mortality of eggs immersed in 1 × 108 conidia ml–1 being 77.4% and 89.7%, respectively. The strain 3297 showed also good aptitudes for stinkbugs control with mortalities higher than 80% against nymphs and adults and eggs mortalities of 75.5% for E. heros and 79.6% for D. furcatus. This study has shown that it is possible to have a two-pronged control strategy, targeting adults and to reduce oviposition and targeting egg clusters to prevent emergence and dispersal of nymphs. Besides early instars of nymphs have been shown to be more susceptible to the fungal strains than late instars and adults.

中文翻译：

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Metarhizium brunneum (Ascomycota: Hypocreales) 菌株对 Stinkbugs Euschistus heros 和 Dichelops furcatus（半翅目：Pentatomidae）的毒力

摘要 对新热带棕色臭虫 Euschistus heros (F.) 和绿腹臭虫 Dichelops furcatus (F.) 的成虫和若虫进行了三种属于绿僵菌属的真菌 Sorokīn (ARSEF 4556, ARSEF 3297, 本地菌株) 的检测。 . 毒性最强的菌株 ARSEF 4556 导致 90% 以上的死亡率。2、5龄若虫和成虫浸入1×108分生孢子ml-1后的平均存活时间分别为4.8、5.7和5.2 d。二龄若虫比成虫更易感。二龄和成年 E. hero 的 LC50 值和中位存活时间分别为 1.6 × 107 和 3.1 × 107 分生孢子 ml-1 以及 6 和 8 天。E. heros 的蛋和密切相关的臭虫 D. furcatus，对 ARSEF 4556 高度敏感，浸泡在 1 × 108 分生孢子 ml-1 中的鸡蛋的平均死亡率分别为 77.4% 和 89.7%。菌株 3297 也表现出良好的控制臭虫的能力，对若虫和成虫的死亡率高于 80%，对 E. heros 和 D. furcatus 的死亡率分别为 75.5% 和 79.6%。这项研究表明，有可能采用双管齐下的控制策略，针对成虫并减少产卵和针对卵群，以防止若虫的出现和扩散。此外，若虫的早龄已被证明比晚龄和成虫更容易受到真菌菌株的影响。英雄和 79.6% 为 D. furcatus。这项研究表明，有可能采用双管齐下的控制策略，针对成虫并减少产卵和针对卵群，以防止若虫的出现和扩散。此外，若虫的早龄已被证明比晚龄和成虫更容易受到真菌菌株的影响。英雄和 79.6% 为 D. furcatus。这项研究表明，有可能采用双管齐下的控制策略，针对成虫并减少产卵和针对卵群，以防止若虫的出现和扩散。此外，若虫的早龄已被证明比晚龄和成虫更容易受到真菌菌株的影响。