Abstract

Benzalkonium chloride (BAC) is a cationic surfactant commonly used as a disinfectant, and is discharged into the aquatic environment by various water sources such as wastewater. BAC may also interact with potentially toxic substances such as persistent organic chemicals. Although studies of BAC contamination toxicity and bioaccumulation have been widely reported, the biochemical responses to BAC toxicity remain incompletely understood, and the detailed molecular mechanisms are largely unknown. In this study, two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry-based proteomic approaches were applied to investigate the protein profiles in Oryzias latipes (medaka) chronically exposed to BAC. Fish were exposed to three different concentrations of BAC, 0.05, 0.1, and 0.2 mg/L, for 21 days. A total of 20 proteins involved in the cytoskeleton, the oxidative stress response, the nervous and endocrine systems, signaling pathways, and cellular proteolysis were significantly upregulated by BAC exposure. The proteomic information obtained in the present study will be useful in identification of potential biomarkers for BAC toxicity, and begins to elucidate its molecular mechanisms, providing new insights into the ecotoxicity of BAC.

摘要

苯扎氯铵（BAC）是常用作消毒剂的阳离子表面活性剂，并通过各种水源（如废水）排放到水生环境中。BAC还可能与潜在的有毒物质（例如持久性有机化学物质）相互作用。尽管已经广泛报道了BAC污染毒性和生物蓄积性的研究，但对BAC毒性的生化反应仍未完全了解，详细的分子机理尚不清楚。在这项研究中，二维凝胶电泳（2-DE）和基质辅助的激光解吸/电离串联飞行时间质谱的蛋白质组学方法被用于调查稻米中的蛋白质谱。（medaka）长期接触BAC。将鱼暴露于三种不同浓度的BAC（0.05、0.1和0.2 mg / L）中21天。BAC暴露显着上调了涉及细胞骨架，氧化应激反应，神经和内分泌系统，信号传导途径以及细胞蛋白水解的20种蛋白质。在本研究中获得的蛋白质组学信息将可用于识别BAC毒性的潜在生物标记，并开始阐明其分子机制，为BAC的生态毒性提供新的见解。