ABSTRACT

The experiment was conducted to appraise whether exogenously applied selenium (Se) could alter nutrients acquisition and metabolism to mitigate the toxic effects of salinity in wheat. Seeds of two contrasting wheat (Triticum aestivum L.) cultivars (Auqab-2000 and Seher-2006) were pre-treated with different concentrations (0, 50, 100 µM) of sodium selenate for 12 h. The pots were irrigated with different NaCl concentrations (0 and 150 mM) applied through Hoagland’s nutrient solution before sowing. Salinity reduced fresh and dry mass and grain yield, but the exogenous Se restored the growth and increased grain yield. Further, salt stress decreased the concentrations of K⁺, Ca²⁺, P, N, Fe and Se while increased the concentration of Na⁺ and Cl^‒ in the shoots and roots of wheat. The exogenous Se increased K⁺, Ca²⁺, N, Fe and Se concentrations while decreased Na⁺, Cl⁻ and P, and thus mainly reversed the inhibitory effects of salinity on nutrients uptake. Further, exogenous Se increased the accumulation of proline and glycinebetaine under salinity. Overall, Se-priming proved an effective shotgun technique to mitigate the detrimental effects of salinity possibly through its beneficial effects on tissue-specific ion acquisition and accumulation of osmoprotectants in wheat.

摘要

进行该实验以评估外源施用硒 (Se) 是否可以改变营养物质的获取和代谢，以减轻盐分对小麦的毒性作用。两种对比小麦 ( Triticum aestivum L.) 品种 (Auqab-2000 和 Seher-2006) 的种子用不同浓度 (0, 50, 100 µM) 的硒酸钠预处理 12 小时。在播种前，通过 Hoagland 营养液应用不同的 NaCl 浓度（0 和 150 mM）对盆进行灌溉。盐分降低了鲜干质量和粮食产量，但外源硒恢复了生长并提高了粮食产量。此外，盐胁迫降低了 K ⁺、Ca ²⁺、P、N、Fe 和 Se 的浓度，而增加了 Na ⁺的浓度和Cl ^-的芽和小麦的根。外源Se增加了K ⁺、Ca ²⁺、N、Fe和Se的浓度，降低了Na ⁺、Cl ^-和P的浓度，从而主要逆转了盐分对养分吸收的抑制作用。此外，外源性硒在盐度下增加了脯氨酸和甘氨酸甜菜碱的积累。总的来说，Se-priming 被证明是一种有效的霰弹枪技术，可以通过其对小麦中渗透保护剂的组织特异性离子获取和积累的有益影响来减轻盐度的不利影响。