Abstract

Five flavonoids were isolated from the ethyl acetate fraction of leaves of Sophora secundiflora; formononetin (1), 5-hydroxy-4’-methoxyflavone (2), genistein (3), 5-hydroxy-8-(1-hydroxy-1-methyl-ethyl)-2-(4-hydroxyphenyl)-4H-furo-[2, 3-h]-chromen-4-one (4) and ononin (5). Additionally, LC-ESI-MS/MS analysis of the ethyl acetate fraction of S. secundiflora leaves had led to tentative identification of eighteen compounds. Formononetin, S. tomentosa and S. secundiflora leaves methanolic extract were evaluated in vivo for their neuroprotective activity where formononetin and S. tomentosa showed promising neuroprotective activity with reduction in acetylcholine esterase (AchE) enzyme activity and elevation of acetylcholine (Ach) and glutathione(GSH) brain levels and attenuation of dopamine (DA), nor-adrenaline (NA) and malonedialdehyde (MDA) brain level significantly, However S. secundiflora leaves methanolic extract didn’t attenuate the AchE enzyme activity, DA and NA brain levels.

摘要

从苦参叶片乙酸乙酯组分中分离得到5种黄酮类化合物；芒柄花素(1) , 5-羟基-4'-甲氧基黄酮(2) , 染料木素(3) , 5-羟基-8-(1-羟基-1-甲基-乙基)-2-(4-羟基苯基)-4 H -furo-[2, 3- h ] -chromen -4-one (4)和 ononin (5)。此外，对S. secundiflora叶子的乙酸乙酯部分进行 LC-ESI-MS/MS 分析，初步鉴定了 18 种化合物。芒柄花素，S.毛白杨和S. secundiflora叶甲醇提取物进行了评价体内因为它们的神经保护活性，其中毛芒花素和S. tomentosa显示出有希望的神经保护活性，乙酰胆碱酯酶 (AchE) 酶活性降低，乙酰胆碱 (Ach) 和谷胱甘肽 (GSH) 脑水平升高，多巴胺 (DA) 和去甲肾上腺素减弱。 NA) 和丙二醛 (MDA) 脑水平显着，但是S. secundiflora叶甲醇提取物没有减弱 AchE 酶活性、DA 和 NA 脑水平。