ABSTRACT

We investigated the distribution of connective tissue fibers in diabetic and vitamin C treated diabetic rat spleen. Rats were divided into three groups: group A, control; group B, diabetic; group C, vitamin C treated diabetic. Diabetes was induced by streptozotocin. Vitamin C was administered intragastrically for 21 days. Spleen tissues were examined by light microscopy after staining with Masson’s trichrome, Gomori silver impregnation and van Gieson. In group B, we found accumulation of collagen fibers in the trabeculae, in the capsule and around the central artery and splenic sinusoids. Splenic cord thickening due to fibrosis was observed. Reticular fibers accumulated principally in the white and red pulps of the spleen and focal reticular fiber thickening was observed in the dense fiber areas. Partial elastic fiber rupture was observed among the fibers of the elastic lamina of the arteries in the hilum. By contrast, the distribution of collagen fibers in group C was similar to group A. Collagen fiber accumulation was decreased in group C compared to group B. We found little reticular fiber thickening in group C and elastic fibers maintained their integrity and were better organized than in group B. Our findings suggest that appropriate doses of vitamin C may exert beneficial effects on the structure of the connective tissue fibers in the diabetic spleen.

摘要

我们调查了结缔组织纤维在糖尿病和维生素 C 治疗的糖尿病大鼠脾脏中的分布。大鼠分为三组：A组，对照组；B组，糖尿病患者；C组，维生素C治疗糖尿病。糖尿病是由链脲佐菌素诱发的。维生素 C 胃内给药 21 天。在用 Masson 三色染色、Gomori 银浸渍和 van Gieson 染色后，通过光学显微镜检查脾组织。在 B 组中，我们发现胶原纤维在小梁、关节囊、中央动脉和脾窦周围积聚。观察到由于纤维化导致的脾索增厚。网状纤维主要积聚在脾脏的白色和红色髓内，在致密纤维区观察到局灶性网状纤维增厚。在肺门动脉的弹性层的纤维中观察到部分弹性纤维断裂。相比之下，C 组胶原纤维的分布与 A 组相似。与 B 组相比，C 组的胶原纤维积累减少。我们发现 C 组中几乎没有网状纤维增厚，弹性纤维保持其完整性，并且比 B 组组织得更好。在 B 组中。我们的研究结果表明适当剂量的维生素 C 可能对糖尿病脾脏中的结缔组织纤维结构产生有益影响。