Transcriptional shifts in delignification‐defective mutants of the white‐rot fungus Pleurotus ostreatus,FEBS Letters

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Transcriptional shifts in delignification‐defective mutants of the white‐rot fungus Pleurotus ostreatus
FEBS Letters ( IF 3.0 ) Pub Date : 2020-08-04 , DOI: 10.1002/1873-3468.13890
Hongli Wu ₁ , Takehito Nakazawa ₁ , Atsuki Takenaka ₁ , Rina Kodera ₁ , Ryota Morimoto ₁ , Masahiro Sakamoto ₁ , Yoichi Honda ₁

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White‐rot fungi efficiently degrade lignin and, thus, play a pivotal role in the global carbon cycle. However, the mechanisms of lignin degradation are largely unknown. Recently, mutations in four genes, namely wtr1, chd1, pex1, and gat1, were shown to abrogate the wood lignin‐degrading ability of Pleurotus ostreatus. In this study, we conducted a comparative transcriptome analysis to identify genes that are differentially expressed in ligninolysis‐deficient mutant strains. Putative ligninolytic genes that are highly expressed in parental strains are significantly downregulated in the mutant strains. On the contrary, many putative cellulolytic and xylanolytic genes are upregulated in the chd1‐1, Δpex1, and Δgat1 strains. Identifying transcriptional alterations in mutant strains could provide new insights into the regulatory mechanisms of lignocellulolytic genes in P. ostreatus.

中文翻译：

白腐真菌 Pleurotus ostreatus 脱木质素缺陷突变体的转录变化

白腐真菌有效降解木质素，因此在全球碳循环中发挥着关键作用。然而，木质素降解的机制在很大程度上是未知的。最近，四个基因的突变，即wtr1、chd1、pex1和gat1，被证明可以消除平菇的木质素降解能力。在这项研究中，我们进行了比较转录组分析，以确定在木质素分解缺陷突变株中差异表达的基因。在亲本菌株中高度表达的推定木质素分解基因在突变菌株中显着下调。相反，许多假定的纤维素分解和木聚糖分解基因在 chd1-1、Δpex1 和 Δgat1 菌株中上调。

更新日期：2020-08-04

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