Background

We investigated uterine histopathological and ultrastructural changes in female dogs with pyometra induced by Escherichia coli (E. coli) inoculation using progesterone and/or estradiol.

Methods

Dogs were ovariectomized and classified into six groups: Groups 1–6 corresponding to estradiol treatment followed by progesterone supplementation, progesterone supplementation only, estradiol supplementation only, simultaneous treatment using estradiol and progesterone, similar to Group 1 but with a double dose, and control group, respectively.

Results

Pyometra was successfully induced in Groups 1, 2, 4, and 5, but not in Group 3. An uneven endometrial surface was observed, along with a purulent discharge, bleeding, inflammatory lesions, cystic endometrial hyperplasia (CEH) or cystic endometrial atrophy. Endometrial thickness percentage, uterine wall thickness, and the percentage of endometrial cyst area increased. Endometrial epithelial mushroom-like hyperplasia and the honeycomb-like structure exposed under the epithelium after flaky exfoliation were found, and the glandular epithelial villi became longer or shorter. Mitochondria expansion and increased lysosome were observed. Endoplasmic reticulum dilation and swelling and many inflammatory cells, especially plasma cell infiltration in the stroma, were found.

Conclusions

Endometrial histopathology and ultrastructural changes in affected dogs were accompanied by induction of pyometra, and they were affected by different hormonal patterns and E. coli.

中文翻译：

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使用孕酮和大肠杆菌诱导子宫蓄脓母犬的子宫内膜组织病理学和细胞超微结构。

背景

我们研究了使用孕酮和/或雌二醇接种大肠杆菌（E.coli）诱导的子宫蓄脓母犬的子宫组织病理学和超微结构变化。

方法

狗被切除卵巢并分为六组：第 1-6 组对应于雌二醇治疗，然后补充孕酮，仅补充孕酮，仅补充雌二醇，同时使用雌二醇和孕酮治疗，与第 1 组相似，但剂量加倍，以及对照组， 分别。

结果

子宫蓄脓在第 1、2、4 和 5 组中成功诱导，但在第 3 组中未成功。观察到子宫内膜表面不平整，伴有脓性分泌物、出血、炎症病变、囊性子宫内膜增生 (CEH) 或囊性子宫内膜萎缩。子宫内膜厚度百分比、子宫壁厚度和子宫内膜囊肿面积百分比增加。子宫内膜上皮呈蘑菇样增生，片状剥落后上皮下外露蜂窝状结构，腺上皮绒毛变长或变短。观察到线粒体扩张和溶酶体增加。内质网扩张肿胀，间质内可见大量炎性细胞，尤其是浆细胞浸润。

结论

受累犬的子宫内膜组织病理学和超微结构变化伴随着子宫积脓的诱导，它们受到不同激素模式和大肠杆菌的影响。