The peri-implantation period of pregnancy is critical for conceptus development, implantation, and signaling for establishment of pregnancy. This study evaluated the effects of bisphenol A (BPA) on proliferation, adhesion, and migration of porcine trophectoderm (pTr2) cells, expression of transporters of arginine and synthesis of amino acids. All concentrations of BPA decreased proliferation and adhesion of pTr2 cells after 96 h compared to the control group. Lower concentrations of BPA (1 × 10⁻⁹, 1 × 10^-8, 10^-7M) increased (P < 0.05), but higher concentrations of BPA (1 × 10^-5, 1 × 10^-4 M) decreased migration of pTr2 cells. BPA increased expression of SLC7A1 mRNA at lower concentrations (1 × 10⁻⁹ to 1 × 10^-6M) and SL7A6, another cationic acid transporter, at higher concentrations (1 × 10^-5, 1 × 10^-4 M). BPA also down-regulated the expression of IGF1 and IGF1 receptor at concentrations of 1 × 10^-7 to 1 × 10^-4 M compared to the control group. The expression of mRNAs for aquaporins (AQP) 3 and 4 were reduced at all concentrations of BPA, but at lower concentrations of BPA, (1 × 10⁻⁹ to 1 × 10^-8M) expression of AQP9 mRNA increased and the expression of AQP11 was not affected by BPA (P > 0.05). There was an inhibitory effect of BPA on the release of synthesis of asparagine, threonine, taurine, tryptophan, and ornithine into the culture medium by pTr2 cells. Collectively, BPA adversely affected the expression of transporters for cationic amino acids like arginine, as well as AQPs, IGF1, and IGF1R associated with proliferation, migration, and adhesion of pTr2 cells. Those adverse effects would likely increase pregnancy losses during the peri-implantation period of pregnancy.

怀孕的围着床期对于概念发育、着床和建立妊娠的信号至关重要。本研究评估了双酚 A (BPA) 对猪滋养外胚层 (pTr2) 细胞增殖、粘附和迁移、精氨酸转运蛋白表达和氨基酸合成的影响。与对照组相比，所有浓度的 BPA 在 96 小时后均降低了 pTr2 细胞的增殖和粘附。较低浓度的 BPA (1 × 10 ^-9 , 1 × 10 ^-8 , 10 ^-7 M) 增加 (P < 0.05)，但较高浓度的 BPA (1 × 10 ^-5 , 1 × 10 ^-4 M) 减少迁移pTr2 细胞。BPA 在较低浓度下增加 SLC7A1 mRNA 的表达（1 × 10^-9至 1 × 10 ^-6 M) 和 SL7A6，另一种阳离子酸转运体，浓度较高 (1 × 10 ^-5 , 1 × 10 ^-4 M)。与对照组相比， BPA 还在 1 × 10 ^-7至 1 × 10 ^-4 M的浓度下下调 IGF1 和 IGF1 受体的表达。在所有 BPA 浓度下，水通道蛋白 (AQP) 3 和 4 的 mRNA 表达均降低，但在 BPA 浓度较低时，(1 × 10 ^-9至 1 × 10 ^-8M) AQP9 mRNA的表达增加，AQP11的表达不受BPA的影响(P > 0.05)。BPA对pTr2细胞合成天冬酰胺、苏氨酸、牛磺酸、色氨酸和鸟氨酸释放到培养基中具有抑制作用。总的来说，BPA 对阳离子氨基酸（如精氨酸）以及与 pTr2 细胞增殖、迁移和粘附相关的 AQP、IGF1 和 IGF1R 的转运蛋白的表达产生不利影响。这些不利影响可能会增加怀孕围着床期的流产率。