The aim of this study was to compare the effect of two sources of dietary oils: rapeseed oil (abundant source of oleic acid) and linseed oil (abundant source of linolenic acid) on the activity of polysaccharidases in sheep rumen. The experiment was carried out on six rumen-fistulated sheep. The animals were divided into three sup-groups of two sheep. Each sup-groups received different sequence of three treatments (control, rapeseed or linseed oils), which resulted in three periods of study. Sheep were fed a diet consisting of meadow hay and moderate amount of concentrates content without or with the addition of rapeseed or linseed oils (50 g/day). The dose of added oils allowed to increase fat content in dietary dry matter from 2 to 7%. After the adaptation period (21 days), rumen digesta was collected for 2 consecutive days before and 2, 4 and 8 h after morning feeding to analyze enzymatic activity. Celulolytic activity decreased 2 and 4 h after feeding when sheep were fed diets with rapeseed or linseed oils in comparison to the control diet. Before feeding, pectinolytic activity was lower in sheep fed oils than in control, but this activity 8 h after feeding was the lowest in animals receiving rapeseed oil. Neither of oil supplements influenced xynalolytic, inulinolytic and amylolytic activity in the rumen of sheep. Irrespective of treatment, xylanolytic activity was the lowest 2 and 4 h after feeding, and the highest at sampling at 0 h. No treatment × time interaction for inulinolytic activity was shown. Comparing sampling time points regardless examined treatment, the amylolytic activity was significantly higher after 2 h after feeding than 0 and 4 h after feeding.

Our data suggested that rapeseed or linseed oil administration to sheep rations modified polysaccharidase activities in the rumen. Both oils, rapeseed and linseed, decreased celulolytic activity in sheep rumen. The addition of rapeseed oil to the diets resulted in decreased pectinolytic activity in the rumen.

这项研究的目的是比较两种食用油来源：菜籽油（丰富的油酸来源）和亚麻籽油（丰富的亚麻酸来源）对绵羊瘤胃多糖酶活性的影响。实验是对六只瘤胃羊进行的。将动物分成两只绵羊的三个小组。每个上班族接受三种处理方法不同的顺序（对照，菜籽油或亚麻子油），这导致了三个研究阶段。给绵羊喂饲由草甸干草和适量精矿组成的日粮，不添加或添加菜籽油或亚麻子油（50克/天）。添加油的剂量可使膳食干物质中的脂肪含量从2％增加到7％。适应期（21天）后，连续2天和2天收集瘤胃消化物。早晨喂食后4和8 h分析酶活性。与对照日粮相比，饲喂绵羊日粮中添加了菜籽油或亚麻子油的日粮在饲喂后2和4 h时，纤维素分解活性降低。饲喂前，绵羊饲喂油中的果胶分解活性比对照低，但饲喂后8小时的活性在接受菜籽油的动物中最低。两种油补充剂均不影响绵羊瘤胃中的xalalolytic，inulinolytic和淀粉分解活性。与处理无关，木糖酵解活性在进食后2和4 h最低，在0 h采样时最高。没有显示亚硝化活性的处理×时间相互作用。比较采样时间点，无论检查的处理如何，喂食后2 h的淀粉分解活性明显高于喂食后0和4 h。

我们的数据表明，向绵羊配给的菜籽油或亚麻子油可改善瘤胃中的多糖酶活性。菜籽油和亚麻子油都降低了绵羊瘤胃的溶菌活性。日粮中添加菜籽油导致瘤胃中的果胶分解活性降低。