In response to changes in their environment bacteria need to change both their protein and phospholipid repertoire to match environmental requirements, but the dynamics of bacterial phospholipid composition under different growth conditions is still largely unknown. In the present study, we investigated the phospholipidome of the bacterial pathogen Campylobacter jejuni. Transcription profiling on logarithmic and stationary phase grown cells of the microaerophilic human pathogen C. jejuni using RNA-seq revealed differential expression of putative phospholipid biosynthesis genes. By applying high-performance liquid chromatography tandem–mass spectrometry, we identified 203 phospholipid species representing the first determination of the phospholipidome of this pathogen. We identified nine different phospholipid classes carrying between one and three acyl chains. Phospholipidome analysis on bacteria of different ages (0–5 days) showed rapid changes in the ratio of phospholipids containing ethanolamine, or glycerol as phospholipid head group and in the number of cyclopropane bond containing fatty acids. Oxygen concentration influenced the percentage of lysophospholipids, and cyclo-propane bonds containing acyl chains. We show that large amounts of the phospholipids are lysophospholipids (30–45%), which mutant studies reveal are needed for normal C. jejuni motility at low oxygen conditions. C. jejuni possesses an unusual phospholipidome that is highly dynamic in response to environmental changes.

为了响应环境的变化，细菌需要同时改变其蛋白质和磷脂库以适应环境要求，但是在不同生长条件下细菌磷脂组成的动力学仍然未知。在当前的研究中，我们调查了空肠弯曲菌空肠弯曲菌的磷脂组。使用RNA序列对微需氧人类病原空肠弯曲菌的对数和固定相生长的细胞进行转录谱分析，揭示了假定的磷脂生物合成基因的差异表达。通过应用高效液相色谱串联质谱法，我们鉴定了203种磷脂种类，首次确定了该病原体的磷脂组。我们确定了九个不同的磷脂类别，携带一到三个酰基链。对不同年龄（0-5天）细菌的磷脂分析表明，含乙醇胺或甘油作为磷脂头基的磷脂比例和含脂肪酸的环丙烷键数量迅速变化。氧浓度影响溶血磷脂和含有酰基链的环丙烷键的百分比。我们表明，大量的磷脂是溶血磷脂（30-45％），突变研究表明，低氧条件下正常空肠弯曲菌的活力是必需的。空肠弯曲杆菌拥有一个不寻常的磷脂组，该磷脂组在响应环境变化时具有很高的动态性。