Anaphylaxis is a life-threatening allergic reaction caused by cross-linking of high-affinity IgE antibodies on the surface of mast cells and basophils. Understanding the cellular mechanisms that lead to high-affinity IgE production is required to develop better therapeutics for preventing this severe reaction. A recently discovered population of T follicular helper T_fh13 cells regulates the production of high-affinity IgE in mouse models of allergy and can also be found in patients with allergies with IgE antibodies against food or aeroallergens. Here we describe optimized protocols for identifying T_fh13 cells in both mice and humans.

中文翻译：

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小鼠和人类 Tfh13 细胞的流式细胞术鉴定

过敏反应是由肥大细胞和嗜碱性粒细胞表面的高亲和力 IgE 抗体交联引起的危及生命的过敏反应。需要了解导致高亲和力 IgE 产生的细胞机制，以开发更好的疗法来预防这种严重反应。最近发现的滤泡辅助 T _fh 13 细胞群在过敏小鼠模型中调节高亲和力 IgE 的产生，并且在对食物或空气过敏原具有 IgE 抗体过敏的患者中也发现了这种现象。在这里，我们描述了用于识别小鼠和人类 T _fh 13 细胞的优化方案。