Abstract Bacterial adhesion on material surfaces can be reduced by different type of coatings. In our study a silica surface on which polyelectrolyte multilayers were formed, with various proteins being the terminating layer, was used as a material surface. We examined three different types of proteins: bovine serum albumin (BSA), lysozyme and glucanase. Adhesion experiments were performed with two bacterial strains: Escherichia coli and Pseudomonas aeruginosa. In order to characterize the surface prior to bacterial adhesion, surface roughness and hydrophobicity were determined by profilometry, atomic force microscopy (AFM) and tensiometry, whereas the surface charge was estimated by zeta potential measurements of silica particles covered with polyelectrolyte multilayers and proteins. The extent of adhered bacteria was examined using scanning electron microscopy (SEM). It was confirmed that in the case where lysozyme was the outermost layer the lysis takes place, whereas in the case of BSA and glucanase specific protein-bacteria interactions are dominant. We showed that the adhesion strongly depends on the protein specificity and the surface physical properties do not play the key role in the adhesion process.

中文翻译：

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蛋白质终止聚电解质多层膜的细菌粘附能力

摘要 不同类型的涂层可以减少细菌在材料表面的粘附。在我们的研究中，将形成聚电解质多层的二氧化硅表面用作终止层，将各种蛋白质用作材料表面。我们检测了三种不同类型的蛋白质：牛血清白蛋白 (BSA)、溶菌酶和葡聚糖酶。用两种细菌菌株进行粘附实验：大肠杆菌和铜绿假单胞菌。为了在细菌粘附之前表征表面，通过轮廓测定法、原子力显微镜 (AFM) 和张力测定法确定表面粗糙度和疏水性，而通过对覆盖有聚电解质多层膜和蛋白质的二氧化硅颗粒的 zeta 电位测量来估计表面电荷。使用扫描电子显微镜（SEM）检查粘附细菌的程度。已经证实，在溶菌酶是最外层的情况下，发生裂解，而在 BSA 和葡聚糖酶的情况下，特异性蛋白质-细菌相互作用占主导地位。我们表明粘附在很大程度上取决于蛋白质的特异性，并且表面物理特性在粘附过程中并不起关键作用。