DNA has been used in the construction of dynamic DNA devices that can reconfigure in the presence of external stimuli. These nanodevices have found uses in fields ranging from biomedical to materials science applications. Here, we report a DNA nanoswitch that can be reconfigured using ribonucleases (RNases) and explore two applications: biosensing and molecular computing. For biosensing, we show the detection of RNase H and other RNases in relevant biological fluids and temperatures, as well as inhibition by the known enzyme inhibitor kanamycin. For molecular computing, we show that RNases can be used to enable erasing, write protection, and erase-rewrite functionality for information-encoding DNA nanoswitches. The simplistic mix-and-read nature of the ribonuclease-activated DNA nanoswitches could facilitate their use in assays for identifying RNase contamination in biological samples or for the screening and characterization of RNase inhibitors.

DNA已用于构建动态DNA设备，该设备可以在外部刺激下重新配置。这些纳米器件已在生物医学到材料科学应用领域中找到了用途。在这里，我们报告了可以使用核糖核酸酶（RNases）进行重组的DNA纳米开关，并探讨了两种应用：生物传感和分子计算。对于生物传感，我们显示了在相关生物流体和温度中对RNase H和其他RNase的检测，以及已知酶抑制剂卡那霉素的抑制作用。对于分子计算，我们表明RNase可用于为信息编码DNA纳米开关提供擦除，写保护和擦除-重写功能。