Protein structure is an important field of research, with particular significance in its potential applications in biomedicine and nanotechnology. In a recent study, we presented a general approach for comparing protein structures and origami models and demonstrated it with single-domain proteins. For example, the analysis of the α-helical barrel of the outer membrane protein A (OmpA) suggests that there are similar patterns between its structure and the Kresling origami model, providing insight into structure-activity relationships. Here we demonstrate that our approach can be expanded beyond single-domain proteins to also include multi-domain proteins, and to study dynamic processes of biomolecules. Two examples are given: (1) The eukaryotic chaperonin (TRiC) protein is compared with a newly generated origami model, and with an origami model that is constructed from two copies of the Flasher origami model, and (2) the CorA Magnesium transport system is compared with a newly generated origami model and with an origami model that combines the Kresling and Flasher origami models. Based on the analysis of the analog origami models, it is indicated that it is possible to identify building blocks for constructing assembled origami models that are analogous to protein structures. In addition, it is identified that the expansion/collapse mechanisms of the TRiC and CorA are auxetic. Namely, these proteins require a single motion for synchronized folding along two or three axes.

蛋白质结构是重要的研究领域，对其在生物医学和纳米技术中的潜在应用特别重要。在最近的研究中，我们提出了一种比较蛋白质结构和折纸模型的通用方法，并用单域蛋白质进行了证明。例如，对外膜蛋白A（OmpA）的α螺旋桶的分析表明，其结构与Kresling折纸模型之间存在相似的模式，从而洞察了结构与活性之间的关系。在这里，我们证明了我们的方法可以从单域蛋白扩展到也包括多域蛋白，并研究生物分子的动态过程。给出了两个例子：（1）将真核伴侣蛋白（TRiC）蛋白与新生成的折纸模型进行比较，并使用由Flasher折纸模型的两个副本构造的折纸模型，以及（2）将CorA镁传输系统与新生成的折纸模型以及结合了Kresling和Flasher折纸模型的折纸模型进行比较。基于对模拟折纸模型的分析，表明可以识别用于构建类似于蛋白质结构的组装折纸模型的构件。此外，已确定TRiC和CorA的扩增/折叠机制是促生长的。即，这些蛋白质需要单个运动以沿着两个或三个轴同步折叠。（2）将CorA镁运输系统与新生成的折纸模型以及结合了Kresling和Flasher折纸模型的折纸模型进行了比较。基于对模拟折纸模型的分析，表明可以识别用于构建类似于蛋白质结构的组装折纸模型的构件。此外，已确定TRiC和CorA的扩增/折叠机制是促生长的。即，这些蛋白质需要单个运动以沿着两个或三个轴同步折叠。（2）将CorA镁运输系统与新生成的折纸模型以及结合了Kresling和Flasher折纸模型的折纸模型进行了比较。基于对模拟折纸模型的分析，表明可以识别用于构建类似于蛋白质结构的组装折纸模型的构件。此外，已确定TRiC和CorA的扩增/折叠机制是促生长的。即，这些蛋白质需要单个运动以沿着两个或三个轴同步折叠。可以确定，TRiC和CorA的扩增/折叠机制是促生长的。即，这些蛋白质需要单个运动以沿着两个或三个轴同步折叠。可以确定，TRiC和CorA的扩增/折叠机制是促生长的。即，这些蛋白质需要单个运动以沿着两个或三个轴同步折叠。