Fluoxetine is a widely prescribed antidepressant that has been frequently detected in aquatic environments and is associated with a series of neurological, behavioural and neuroendocrine disruptions in nontarget organisms. However, studies on its effects in fish under realistic environmental conditions are still limited. In this study, we determined the influences of an environmentally relevant concentration of fluoxetine (100 ng/L) on crucian carp (Carassius auratus) in the presence of dissolved organic matter (DOM). Endpoints that were assessed included accumulation of fluoxetine and metabolite formation as well as related biological responses involving neurotransmission and metabolic processes. Fluoxetine was significantly bioconcentrated in the fish brain and liver and largely transformed to the active metabolite norfluoxetine. Brain neurotransmission processes related to serotonin and choline and liver metabolic status were simultaneously altered. DOM added at 1 mg/L had no effect on the accumulation of fluoxetine or its metabolites in different tissues of the fish. However, at 10 mg/L DOM facilitated fluoxetine and norfluoxetine accumulation in the liver, brain, kidney, gill and bile tissues of the fish. The neuroendocrine-disrupting effects on fish caused by fluoxetine were also enhanced by the co-addition of DOM at 10 mg/L. Binding with fluoxetine and the inhibition of metabolic functions caused by DOM may be responsible for this increase in effects. These findings imply that at high concentrations DOM can increase the toxicity of environmentally relevant concentrations of fluoxetine to fish.

氟西汀是一种处方广泛的抗抑郁药，在水生环境中经常被发现，并且与非靶标生物中的一系列神经，行为和神经内分泌干扰有关。但是，在现实环境条件下对鱼类的影响的研究仍然有限。在这项研究中，我们确定了环境相关浓度的氟西汀（100 ng / L）对cru鱼（Carassius auratus）的影响。）存在于溶解的有机物（DOM）中。评估的终点包括氟西汀的积累和代谢产物的形成，以及涉及神经传递和代谢过程的相关生物学反应。氟西汀在鱼脑和肝脏中大量生物富集，并大量转化为活性代谢物去氟西汀。与5-羟色胺和胆碱及肝脏代谢状态有关的脑神经传递过程同时改变。以1 mg / L添加的DOM对鱼的不同组织中氟西汀或其代谢产物的积累没有影响。然而，在10 mg / L的DOM下，氟西汀和去氟西汀在鱼的肝脏，脑，肾脏，g和胆汁组织中的蓄积变得容易。氟西汀对鱼的神经内分泌干扰作用也因10 mg / L DOM的共添加而增强。与氟西汀的结合以及由DOM引起的代谢功能的抑制可能是这种作用增加的原因。这些发现表明，高浓度的DOM可以增加与环境有关的氟西汀浓度对鱼类的毒性。