There has been development of an antiretrograde flow device (DARIO), for sheep cervical artificial insemination (CAI). There, however, needs to be optimization of sperm volume and concentration of insemination doses when the DARIO is used for CAI. Objectives were to compare fertility rates (proportion of ewes lambing as a result of CAI) when there was use of the DARIO for CAI: two sperm volumes containing equal numbers of spermatozoa: 0.25 mL of 1,600 × 10⁶ spermatozoa/mL and 0.50 mL of 800 × 10⁶ spermatozoa/mL (Test 1 group), and two sperm volumes with a different number of spermatozoa/AI dose: 0.25 mL and 0.50 mL of 1,600 × 10⁶ spermatozoa/mL (Test 2 group). There were 335 ewes from seven farms assigned to 60 batches (equally divided into a Control and Test 1 group). For the Test 2 group, 462 ewes from nine farms were assigned to 88 batches (equally proportioned into Control group and Test 2 groups). For the Test 1 group, proportion of ewes lambing as a result of CAI were 0.701 ± 0.2679 and 0.595 ± 0.2393 for the Control and Test 1 groups, respectively (P = 0.163). For the Test 2 group, proportions of ewes lambing were 0.550 ± 0.2598 and 0.658 ± 0.2412 for the Control and Test 2 group, respectively (P = 0.041). An inclusion of a larger number of spermatozoa per insemination in a 0.50 mL dose volume resulted improved proportion of ewes lambing as a result of CAI when there was used of the DARIO.

已经开发出一种用于羊宫颈人工授精 (CAI) 的抗逆流装置 (DARIO)。然而，当 DARIO 用于 CAI 时，需要优化精子体积和授精剂量浓度。目的是比较使用 DARIO 进行 CAI 时的生育率（由于 CAI 产羔的母羊比例）：两个精子体积包含相同数量的精子：0.25 mL 的 1,600 × 10 ⁶精子/mL 和 0.50 mL 800 × 10 ⁶精子/mL（试验1组），和两个精子体积不同数量的精子/AI剂量：0.25 mL和0.50 mL 1,600 × 10 ⁶精子/毫升（测试 2 组）。有来自七个农场的 335 只母羊被分配到 60 个批次（均分为对照组和测试 1 组）。对于测试 2 组，来自 9 个农场的 462 只母羊被分配到 88 个批次（等比例分为对照组和测试 2 组）。对于测试 1 组，由于 CAI 导致的母羊产羔比例分别为 0.701 ± 0.2679 和 0.595 ± 0.2393（对照组和测试 1 组）（P = 0.163）。对于测试 2 组，对照组和测试 2 组的母羊产羔比例分别为 0.550 ± 0.2598 和 0.658 ± 0.2412 ( P = 0.041)。当使用 DARIO 时，由于 CAI，在 0.50 mL 剂量体积中每次授精包含更多数量的精子导致母羊产羔的比例提高。