Fibroblasts produce collagen which is mainly essential for repairing tissue damage and maintaining the structural integrity of tissues. However, studies have given scientific evidence about harmful effect of thermal manipulation in fibroblast. Therefore, the aim of this study was to determine the mild heat stress temperature which increased broiler fibroblast viability. The experiment was divided into two groups (37 °C and 41 °C), and each group was divided into five subgroups based on different incubation times (6 h, 12 h, 24 h, 48 h, and 72 h) with three replications. In experimental group (41 °C), fibroblast viability increased significantly in 12 h but decreased in 72 h compared with control (37 °C). At 41 °C, live cell increased significantly in 24 h and then declined in 48 h as well as 72 h than control. Moreover, the S phase lengthened in shorter incubation time of experimental group compared with control. Protein and mRNA (HSP70, HSP60, and HSP47) expressions were significantly higher at 41 °C compared with 37 °C, but at the end of the experiment, HSP expression level was higher in both groups. Finally, this study recommended 41 °C as a mild heat stress temperature for increasing broiler fibroblast viability.

成纤维细胞产生胶原蛋白，这对于修复组织损伤和维持组织结构完整性至关重要。然而，研究已经提供了关于热操作对成纤维细胞有害影响的科学证据。因此，本研究的目的是确定提高肉鸡成纤维细胞活力的轻度热应激温度。实验分为两组（37℃和41℃），每组根据不同孵育时间（6 h、12 h、24 h、48 h、72 h）又分为5个亚组，3个重复。与对照组（37℃）相比，实验组（41℃）成纤维细胞活力在12小时内显着增加，但在72小时内下降。在41℃下，与对照相比，活细胞在24小时内显着增加，然后在48小时和72小时内下降。此外，与对照组相比，实验组的S期延长，孵化时间更短。与37℃相比，41℃时蛋白质和mRNA（HSP70、HSP60和HSP47）的表达显着升高，但在实验结束时，两组的HSP表达水平均较高。最后，本研究建议将 41 °C 作为提高肉鸡成纤维细胞活力的温和热应激温度。