The antimicrobial resistance of food-borne pathogenic bacteria, including Vibrio parahaemolyticus, has been reported globally, warranting the need to identify promising alternative antibiotics such as endolysins that originate from bacteriophages. In our previous study, we characterized a bacteriophage infecting V. parahaemolyticus, vB_VpaP_KF2, at the molecular level. In this study, an open reading frame encoding putative endolysin was cloned from the complete genome data and expressed in the Escherichia coli expression system. The recombinant endolysin, vB_VpaP_KF2_Lys, exhibited a novel lytic property against Gram-negative bacteria regardless of pretreatment with an outer-membrane permeabilizer. It was also stable over a wide range of temperatures, pH, and NaCl concentrations, and its hydrolytic spectrum was broader than that of the parent bacteriophage. From the results, vB_VpaP_KF2_Lys could be used as a biocontrol agent against food-borne pathogens in the field of food safety.

中文翻译：

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从噬菌体感染副溶血性弧菌vB_VpaP_KF2的新型溶素

食源性致病细菌（包括副溶血性弧菌）的耐药性已在全球范围内得到报道，因此有必要确定有前途的替代抗生素，例如源自噬菌体的溶血素。在我们之前的研究中，我们在分子水平上鉴定了感染副溶血性弧菌vB_VpaP_KF2的噬菌体。在这项研究中，从完整的基因组数据中克隆了编码推定的细胞内溶素的开放阅读框，并在大肠杆菌表达系统中表达。重组内皮细胞溶素，vB_VpaP_KF2_Lys对革兰氏阴性细菌显示出新颖的裂解特性，而无需使用外膜透化剂进行预处理。在广泛的温度，pH和NaCl浓度范围内，它也是稳定的，其水解谱比亲本噬菌体宽。从结果可以看出，vB_VpaP_KF2_Lys可用作食品安全领域中食源性病原体的生物防治剂。