Multiplexed assays allow functional testing of large synthetic libraries of genetic elements, but are limited by the designability, length, fidelity and scale of the input DNA. Here, we improve DropSynth, a low-cost, multiplexed method that builds gene libraries by compartmentalizing and assembling microarray-derived oligonucleotides in vortexed emulsions. By optimizing enzyme choice, adding enzymatic error correction and increasing scale, we show that DropSynth can build thousands of gene-length fragments at >20% fidelity.

中文翻译：

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DropSynth 2.0：乳液中的高保真多重基因合成。

多重测定允许对大型合成的遗传元件文库进行功能测试，但受到输入DNA的可设计性，长度，保真度和规模的限制。在这里，我们改进了DropSynth，这是一种低成本的多路复用方法，可通过在涡旋乳液中分隔和组装微阵列衍生的寡核苷酸来构建基因库。通过优化酶的选择，增加酶学误差校正和增加规模，我们证明了DropSynth可以以> 20％的保真度构建成千上万的基因长度片段。