Abstract

This work aimed to explore optimal conditions for keratose (KR), collagen (CL), and KR/CL blend microparticles preparation using a water-in-oil emulsion solvent diffusion method. The protein solution was used as the water phase (W) and ethyl acetate was used as the oil phase (O). Scanning electron microscopy (SEM) results indicated that most of the KR microparticles were hollow, with a rough and dented outer surface while CL microparticles had variable shapes with smooth surfaces and some small pores distributed inside the particles. Morphology of the KR/CL blend microparticles varied according to types of polymer, mass ratio, and the condition used. All particles had as main structure, an α-helix co-existing with random coil structures. The decomposition steps of the KR and CL microparticles followed similar trends while the Td, max of blend particles varied at different mass ratios of KR and KL. The high content of CL influenced the formation of a dense texture of the blend microparticles. The prepared microparticles showed a drug-controlled release profile that could be applied as a drug delivery system.

摘要

这项工作旨在探索使用油包水乳液溶剂扩散法制备角糖 (KR)、胶原蛋白 (CL) 和 KR/CL 混合微粒的最佳条件。蛋白质溶液用作水相（W），乙酸乙酯用作油相（O）。扫描电子显微镜（SEM）结果表明，大多数 KR 微粒是中空的，外表面粗糙和凹陷，而 CL 微粒形状各异，表面光滑，颗粒内部分布有一些小孔。KR/CL 共混物微粒的形态因聚合物类型、质量比和使用条件而异。所有粒子都具有作为主要结构的α-螺旋与无规卷曲结构共存。KR 和 CL 微粒的分解步骤遵循相似的趋势，而 Td，混合粒子的最大值在 KR 和 KL 的不同质量比下变化。高含量的 CL 影响了共混微粒的致密质地的形成。制备的微粒显示出可用作药物递送系统的药物控释曲线。