Abstract

A novel alkaloid identified as methyl 8,9-dihydroxy-11-oxo-6,11-dihydro-5H-benzo[d]pyrrolo[1,2-a]azepine-3-carboxylate, named portulacatone A (1) with six known compounds Oleracein E (2), 6,7-dihdroxy-3,4-dihydro-2H-isoquinolin-1-one (3), N-trans-p-coumaroytyramine (4), 9H-carbazole (5), isoaspergin (6) and flavoglaucin (7) were obtained from Portulaca oleracea L., while compounds (5–7) were isolated from the plant for the first time. The new structure was identified by using UHPLC-ESI-Q-TOF/MS, 1D, 2D NMR and the others were proved by ¹H-NMR and ¹³C NMR that comparing with previous reports. It was suggested that the portulacatone A (1) can significantly inhibit the inflammatory factor, interleukin-1β (IL-1β) in the RAW 264.7 cells induced by LPS.

摘要

一种新的生物碱，被鉴定为甲基 8,9-dihydroxy-11-oxo-6,11-dihydro-5H-benzo[d]pyrrolo[1,2-a]azepine-3-carboxylate，命名为 portulacatone A ( 1 )，含有六个已知化合物橄榄苦苷 E ( 2 ), 6,7-dihdroxy-3,4-dihydro-2H-isoquinolin-1-one ( 3 ), N - trans - p-香豆酪胺 ( 4 ), 9H-咔唑 ( 5 ), 异天冬氨酸( 6 ) 和flavoglaucin ( 7 ) 从马齿苋中获得，而化合物( 5-7) 首次从植物中分离出来。新结构采用UHPLC-ESI-Q-TOF/MS、1D、2D NMR鉴定，其他结构经¹ H-NMR和¹³ C NMR证实，与以往报道比较。提示portulacatone A ( 1 ) 可显着抑制LPS诱导的RAW 264.7细胞中的炎症因子白细胞介素-1β(IL-1β)。