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Loss of GFAP and Vimentin Does Not Affect Peri-Infarct Depolarizations after Focal Cerebral Ischemia
European Neurology ( IF 2.1 ) Pub Date : 2020-01-01 , DOI: 10.1159/000507990
Zelong Zheng 1 , Xuxia Yi 1 , Jianping Lv 2
Affiliation  

Peri-infarct depolarization (PID), one kind of spreading depolarization, contributes to infarct volume enlargement after ischemic stroke. Astrocytes participate in PIDs by various mechanisms. The roles of glial fibrillary acidic protein (GFAP) and vimentin (Vim), intermediate filament proteins in astrocytes, however, in PIDs induction and propagation remain unknown. Middle cerebral artery occlusion (MCAO) model was made in 9 GFAP−/−Vim−/− and 9 wild-type (WT) C57BL/6 mice. Using 4-wavelength optical intrinsic signal imaging (OIS), we identified PIDs as consistent, red and blue interaction waves in the cortical reflectance that slowly propagated peripherally from the origin site. Five propagation patterns of PIDs were observed after MCAO in mice, namely, latero-medial, medial-lateral, rostro-caudal, caudo-rostral, and collision. Additionally, the frequency, propagation velocity, and duration of PIDs between GFAP−/−Vim−/− and WT mice were not significantly different (p > 0.05). Furthermore, no significant difference was found in infarct volume and brain edema between the two groups. In conclusion, the 4-wavelength OIS system allows acquisition of high temporal-spatial resolution color images for analyzing temporal-spatial characteristics of PIDs in detail. GFAP and Vim in astrocytes are not involved in PIDs after MCAO in mice.

中文翻译:

GFAP 和波形蛋白的丢失不影响局灶性脑缺血后梗塞周围去极化

梗死周围去极化(PID)是一种扩散去极化,有助于缺血性卒中后梗死体积扩大。星形胶质细胞通过各种机制参与 PID。然而,胶质纤维酸性蛋白 (GFAP) 和波形蛋白 (Vim),星形胶质细胞中的中间丝蛋白,在 PID 诱导和传播中的作用仍然未知。在 9 只 GFAP-/-Vim-/- 和 9 只野生型 (WT) C57BL/6 小鼠中制作大脑中动脉闭塞 (MCAO) 模型。使用 4 波长光学固有信号成像 (OIS),我们将 PID 识别为皮质反射中一致的红色和蓝色相互作用波,这些波从原点向外缓慢传播。在小鼠 MCAO 后观察到 PIDs 的五种传播模式, 即外侧内侧、内侧外侧、rostro-caudal、caudo-rostral 和碰撞。此外,GFAP-/-Vim-/- 和 WT 小鼠之间 PID 的频率、传播速度和持续时间没有显着差异(p > 0.05)。此外,两组之间的梗死体积和脑水肿没有显着差异。总之,4 波长 OIS 系统允许获取高时空分辨率彩色图像,用于详细分析 PID 的时空特性。小鼠 MCAO 后星形胶质细胞中的 GFAP 和 Vim 不参与 PID。4 波长 OIS 系统允许获取高时空分辨率彩色图像,用于详细分析 PID 的时空特性。小鼠 MCAO 后星形胶质细胞中的 GFAP 和 Vim 不参与 PID。4 波长 OIS 系统允许获取高时空分辨率彩色图像,用于详细分析 PID 的时空特性。小鼠 MCAO 后星形胶质细胞中的 GFAP 和 Vim 不参与 PID。
更新日期:2020-01-01
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