With time-resolved crystallography (TRX), it is possible to follow the reaction dynamics in biological macromolecules by investigating the structure of transient states along the reaction coordinate. X-ray free electron lasers (XFELs) have enabled TRX experiments on previously uncharted femtosecond timescales. Here, we review the recent developments, opportunities, and challenges of pump-probe TRX at XFELs.

中文翻译：

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X射线自由电子激光器的泵浦时间分辨串联飞秒晶体学

使用时间分辨晶体学（TRX），可以通过研究沿反应坐标的瞬态结构来跟踪生物大分子中的反应动力学。X射线自由电子激光器（XFEL）使TRX实验能够在以前未知的飞秒时间范围内进行。在这里，我们回顾了XFEL的泵探头TRX的最新发展，机遇和挑战。