A novel straightforward analytical technique was developed to monitor the emission of hydrogen from anaerobic bacteria cultured in sealed headspace vials using a specific hydrogen sensor. The results were compared with headspace gas chromatography carried out in parallel. This technique was also applied to investigate the efficacy of chemical antibiotics and of natural compounds with antimicrobial properties. Antibiotics added to the sample cultures are apparently effective if the emission of hydrogen is suppressed, or if not, are either ineffective or the related bacteria are even resistant. The sensor approach was applied to prove bacterial contamination in food, animals, medical specimens and in ticks infected by Borrelia bacteria and their transfer to humans, thus causing Lyme disease. It is a unique advantage that the progress of an antibiotic therapy can be examined until the emission of hydrogen is finished. The described technique cannot identify the related bacteria but enables bacterial contamination by hydrogen emitting anaerobes to be recognized. The samples are incubated with the proper culture broth in closed septum vials which remain closed during the whole process. The personnel in the lab never come into contact with pathogens and thus safety regulations are guaranteed.

中文翻译：

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通过特定的氢传感器监测食物，动物和患有莱姆病的人血液中细菌的氢排放。

开发了一种新颖的简单分析技术，以使用特定的氢传感器监测密封在顶空小瓶中培养的厌氧细菌的氢排放。将结果与并行进行的顶空气相色谱法进行比较。该技术还用于研究化学抗生素和具有抗菌特性的天然化合物的功效。如果抑制了氢的排放，则添加到样本培养物中的抗生素显然是有效的，或者如果无效，或者是无效的，或者相关细菌甚至具有抗性。应用传感器方法来证明食物，动物，医学标本和被博雷利亚感染的tick虫中的细菌污染细菌及其向人类的转移，从而导致莱姆病。一个独特的优势是可以检查抗生素治疗的进展，直到完成氢的释放为止。所描述的技术不能识别相关细菌，但是能够识别出氢排放厌氧菌对细菌的污染。将样品与合适的培养液一起放在封闭的隔垫小瓶中孵育，在整个过程中均保持封闭状态。实验室人员永远不会接触病原体，因此可以保证安全性。